Prostacyclin receptor agonists induce DUSP1 to inhibit pulmonary artery smooth muscle cell proliferation.


Journal

Life sciences
ISSN: 1879-0631
Titre abrégé: Life Sci
Pays: Netherlands
ID NLM: 0375521

Informations de publication

Date de publication:
15 Feb 2023
Historique:
received: 09 10 2022
revised: 08 12 2022
accepted: 02 01 2023
pubmed: 8 1 2023
medline: 1 2 2023
entrez: 7 1 2023
Statut: ppublish

Résumé

Upregulated p38 PA-SMCs were treated with BMP2, ET-1, prostacyclin, and MRE-269, an active metabolite of selexipag, either alone or in combination. We quantified mRNA expressions using real-time quantitative polymerase chain reaction. Pulmonary artery specimens and PA-SMCs were obtained during lung transplantation in patients with PAH. Both prostacyclin and MRE-269 increased DUSP1 expression. Combined treatment with BMP2 and ET-1 induced cyclin D1 and DUSP1 expression and increased PA-SMC proliferation. MRE-269 attenuated BMP2/ET-1-induced cell proliferation. ET-1 increased DUSP1 expression in PA-SMCs from control patients but not in PA-SMCs from patients with PAH. This study showed that the p38

Identifiants

pubmed: 36608870
pii: S0024-3205(23)00006-1
doi: 10.1016/j.lfs.2023.121372
pii:
doi:

Substances chimiques

selexipag 5EXC0E384L
(4-((5,6-diphenylpyrazin-2-yl)(isopropyl)amino)butoxy)acetic acid E9PC7N0DID
Receptors, Epoprostenol 0
Endothelin-1 0
Prostaglandins I 0
DUSP1 protein, human EC 3.1.3.48
Dual Specificity Phosphatase 1 EC 3.1.3.48

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

121372

Informations de copyright

Copyright © 2023 Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors declare no conflict of interest.

Auteurs

Hidekazu Maruyama (H)

Department of Cardiology, National Hospital Organization Kasumigaura Medical Center, 300-8585 Tsuchiura, Japan; Division of Cardiovascular Medicine, Faculty of Medicine, University of Tsukuba, 305-8577 Tsukuba, Japan; Laboratory of Physiology and Pharmacology, Faculty of Medicine, Université Libre de Bruxelles, 1070 Brussels, Belgium. Electronic address: maruyama.hidekazu.mj@alumni.tsukuba.ac.jp.

Satoshi Sakai (S)

Faculty of Health Science, Tsukuba University of Technology, 305-8520 Tsukuba, Japan.

Laurence Dewachter (L)

Laboratory of Physiology and Pharmacology, Faculty of Medicine, Université Libre de Bruxelles, 1070 Brussels, Belgium.

Céline Dewachter (C)

Laboratory of Physiology and Pharmacology, Faculty of Medicine, Université Libre de Bruxelles, 1070 Brussels, Belgium; Department of Cardiology, Erasme Academic Hospital, 1070 Brussels, Belgium.

Benoit Rondelet (B)

Department of Cardiac, Vascular and Thoracic Surgery, CHU UCL Namur, 5530 Yvoir, Belgium.

Robert Naeije (R)

Laboratory of Physiology and Pharmacology, Faculty of Medicine, Université Libre de Bruxelles, 1070 Brussels, Belgium.

Masaki Ieda (M)

Division of Cardiovascular Medicine, Faculty of Medicine, University of Tsukuba, 305-8577 Tsukuba, Japan.

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Classifications MeSH