Genome sequencing and assembly of Lathyrus sativus - a nutrient-rich hardy legume crop.


Journal

Scientific data
ISSN: 2052-4463
Titre abrégé: Sci Data
Pays: England
ID NLM: 101640192

Informations de publication

Date de publication:
17 01 2023
Historique:
received: 18 06 2022
accepted: 14 12 2022
entrez: 17 1 2023
pubmed: 18 1 2023
medline: 20 1 2023
Statut: epublish

Résumé

Grass pea (Lathyrus sativus) is a cool-season legume crop tolerant to drought, salinity, waterlogging, insects, and other biotic stresses. Despite these beneficial traits, this crop is not cultivated widely due to the accumulation of a neurotoxin - β-N-oxalyl-L-α, β-diaminopropionic acid (β-ODAP) in the seeds and its association with neurolathyrism. In this study, we sequenced and assembled the genome of Lathyrus sativus cultivar Pusa-24, an elite Indian cultivar extensively used in breeding programs. The assembled genome of Lathyrus was 3.80 Gb in length, with a scaffold N50 of 421.39 Mb. BUSCO assessment indicated that 98.3% of highly conserved Viridiplantae genes were present in the assembly. A total of 3.17 Gb (83.31%) of repetitive sequences and 50,106 protein-coding genes were identified in the Lathyrus assembly. The Lathyrus genome assembly reported here thus provides a much-needed and robust foundation for various genetic and genomic studies in this vital legume crop.

Identifiants

pubmed: 36650149
doi: 10.1038/s41597-022-01903-4
pii: 10.1038/s41597-022-01903-4
pmc: PMC9845207
doi:

Substances chimiques

Amino Acids, Diamino 0

Types de publication

Dataset Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

32

Informations de copyright

© 2023. The Author(s).

Références

Campbell, C. et al. in Expanding the production and use of cool season food legumes 617–630 (Springer, 1994).
Croft, A., Pang, E. & Taylor, P. Molecular analysis of Lathyrus sativus L.(grasspea) and related Lathyrus species. Euphytica 107, 167–176 (1999).
doi: 10.1023/A:1003520721375
Kumar, S., Bejiga, G., Ahmed, S., Nakkoul, H. & Sarker, A. Genetic improvement of grass pea for low neurotoxin (β-ODAP) content. Food and Chemical Toxicology 49, 589–600 (2011).
doi: 10.1016/j.fct.2010.06.051
Lambein, F., Travella, S., Kuo, Y.-H., Van Montagu, M. & Heijde, M. Grass pea (Lathyrus sativus L.): orphan crop, nutraceutical or just plain food? Planta 250, 821–838, https://doi.org/10.1007/s00425-018-03084-0 (2019).
doi: 10.1007/s00425-018-03084-0
Galbraith, D. W. et al. Rapid flow cytometric analysis of the cell cycle in intact plant tissues. Science 220, 1049–1051, https://doi.org/10.1126/science.220.4601.1049 (1983).
doi: 10.1126/science.220.4601.1049
Bolger, A. M., Lohse, M. & Usadel, B. Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics 30, 2114–2120, https://doi.org/10.1093/bioinformatics/btu170 (2014).
doi: 10.1093/bioinformatics/btu170
Zimin, A. V. et al. The MaSuRCA genome assembler. Bioinformatics 29, 2669–2677, https://doi.org/10.1093/bioinformatics/btt476 (2013).
doi: 10.1093/bioinformatics/btt476
Zimin, A. V. et al. Hybrid assembly of the large and highly repetitive genome of Aegilops tauschii, a progenitor of bread wheat, with the MaSuRCA mega-reads algorithm. Genome research 27, 787–792, https://doi.org/10.1101/gr.213405.116 (2017).
doi: 10.1101/gr.213405.116
Kreplak, J. et al. A reference genome for pea provides insight into legume genome evolution. Nature Genetics 51, 1411–1422, https://doi.org/10.1038/s41588-019-0480-1 (2019).
doi: 10.1038/s41588-019-0480-1
Alonge, M. et al. RaGOO: fast and accurate reference-guided scaffolding of draft genomes. Genome Biology 20, 224, https://doi.org/10.1186/s13059-019-1829-6 (2019).
doi: 10.1186/s13059-019-1829-6
Bao, W., Kojima, K. K. & Kohany, O. Repbase Update, a database of repetitive elements in eukaryotic genomes. Mobile DNA 6, 11, https://doi.org/10.1186/s13100-015-0041-9 (2015).
doi: 10.1186/s13100-015-0041-9
Tarailo-Graovac, M. & Chen, N. Using RepeatMasker to identify repetitive elements in genomic sequences. Current protocols in bioinformatics Chapter 4, Unit 4, 10, https://doi.org/10.1002/0471250953.bi0410s25 (2009).
doi: 10.1002/0471250953.bi0410s25
Bruna, T., Hoff, K. J., Lomsadze, A., Stanke, M. & Borodovsky, M. BRAKER2: automatic eukaryotic genome annotation with GeneMark-EP+ and AUGUSTUS supported by a protein database. NAR genomics and bioinformatics 3, lqaa108, https://doi.org/10.1093/nargab/lqaa108 (2021).
doi: 10.1093/nargab/lqaa108
Verma, A. et al. Contrasting β-ODAP content correlates with stress gene expression in Lathyrus cultivars. Physiologia Plantarum 174, e13616, https://doi.org/10.1111/ppl.13616 (2022).
doi: 10.1111/ppl.13616
NCBI Sequence Read Archive https://identifiers.org/ncbi/insdc.sra:SRX15778696 (2022).
NCBI Sequence Read Archive https://identifiers.org/ncbi/insdc.sra:SRX14424107 (2022).
NCBI Sequence Read Archive https://identifiers.org/ncbi/insdc.sra:SRX14424109 (2022).
NCBI Sequence Read Archive https://identifiers.org/ncbi/insdc.sra:SRX14424110 (2022).
NCBI Sequence Read Archive https://identifiers.org/ncbi/insdc.sra:SRX14424106 (2022).
NCBI Sequence Read Archive https://identifiers.org/ncbi/insdc.sra:SRX14424108 (2022).
NCBI Sequence Read Archive https://identifiers.org/ncbi/insdc.sra:SRX14424111 (2022).
Rajarammohan, S. et al. Lathyrus sativus Pusa-24, whole genome shotgun sequencing project. GenBank https://identifiers.org/nucleotide:JAPMLZ000000000 (2022).
Rajarammohan, S. et al. Genome sequencing and assembly of Lathyrus sativus (Dataset). zenodo https://doi.org/10.5281/zenodo.7297773 (2022).
Emmrich, P. M. F. et al. A draft genome of grass pea (Lathyrus sativus), a resilient diploid legume. bioRxiv, 2020.2004.2024.058164, https://doi.org/10.1101/2020.04.24.058164 (2020).
Simao, F. A., Waterhouse, R. M., Ioannidis, P., Kriventseva, E. V. & Zdobnov, E. M. BUSCO: assessing genome assembly and annotation completeness with single-copy orthologs. Bioinformatics 31, 3210–3212, https://doi.org/10.1093/bioinformatics/btv351 (2015).
doi: 10.1093/bioinformatics/btv351
Xu, Q., Liu, F., Chen, P., Jez, J. M. & Krishnan, H. B. β-N-Oxalyl-l-α,β-diaminopropionic Acid (β-ODAP) Content in Lathyrus sativus: The Integration of Nitrogen and Sulfur Metabolism through β-Cyanoalanine Synthase. International Journal of Molecular Sciences 18, 526 (2017).
doi: 10.3390/ijms18030526

Auteurs

Sivasubramanian Rajarammohan (S)

National Agri-Food Biotechnology Institute, Mohali, 140306, India.

Lovenpreet Kaur (L)

National Agri-Food Biotechnology Institute, Mohali, 140306, India.

Anjali Verma (A)

National Agri-Food Biotechnology Institute, Mohali, 140306, India.

Dalwinder Singh (D)

National Agri-Food Biotechnology Institute, Mohali, 140306, India.

Shrikant Mantri (S)

National Agri-Food Biotechnology Institute, Mohali, 140306, India.

Joy K Roy (JK)

National Agri-Food Biotechnology Institute, Mohali, 140306, India.

Tilak Raj Sharma (TR)

National Agri-Food Biotechnology Institute, Mohali, 140306, India.
Division of Crop Sciences, Indian Council of Agricultural Research, Krishi Bhawan, New Delhi, 110001, India.

Ashwani Pareek (A)

National Agri-Food Biotechnology Institute, Mohali, 140306, India.

Pramod Kaitheri Kandoth (PK)

National Agri-Food Biotechnology Institute, Mohali, 140306, India. pramod@nabi.res.in.
Department of Plant Sciences, Central University of Kerala, Kasaragod, 670320, India. pramod@nabi.res.in.

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