Molecular diagnosis and novel genes and phenotypes in a pediatric thoracic insufficiency cohort.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
18 01 2023
Historique:
received: 26 05 2022
accepted: 05 01 2023
entrez: 18 1 2023
pubmed: 19 1 2023
medline: 21 1 2023
Statut: epublish

Résumé

Thoracic insufficiency syndromes are a genetically and phenotypically heterogeneous group of disorders characterized by congenital abnormalities or progressive deformation of the chest wall and/or vertebrae that result in restrictive lung disease and compromised respiratory capacity. We performed whole exome sequencing on a cohort of 42 children with thoracic insufficiency to elucidate the underlying molecular etiologies of syndromic and non-syndromic thoracic insufficiency and predict extra-skeletal manifestations and disease progression. Molecular diagnosis was established in 24/42 probands (57%), with 18/24 (75%) probands having definitive diagnoses as defined by laboratory and clinical criteria and 6/24 (25%) probands having strong candidate genes. Gene identified in cohort patients most commonly encoded components of the primary cilium, connective tissue, and extracellular matrix. A novel association between KIF7 and USP9X variants and thoracic insufficiency was identified. We report and expand the genetic and phenotypic spectrum of a cohort of children with thoracic insufficiency, reinforce the prevalence of extra-skeletal manifestations in thoracic insufficiency syndromes, and expand the phenotype of KIF7 and USP9X-related disease to include thoracic insufficiency.

Identifiants

pubmed: 36653407
doi: 10.1038/s41598-023-27641-0
pii: 10.1038/s41598-023-27641-0
pmc: PMC9849333
doi:

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

991

Subventions

Organisme : NIDDK NIH HHS
ID : K08 DK128606
Pays : United States

Informations de copyright

© 2023. The Author(s).

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Auteurs

Alanna Strong (A)

Division of Human Genetics, Children's Hospital of Philadelphia, Philadelphia, PA, USA.
The Center for Applied Genomics, Children's Hospital of Philadelphia, Philadelphia, PA, USA.
Department of Pediatrics, Perelman School of Medicine, Children's Hospital of Philadelphia, University of Pennsylvania, 3615 Civic Center Blvd, Philadelphia, PA, 19104, USA.

Meckenzie Behr (M)

The Center for Applied Genomics, Children's Hospital of Philadelphia, Philadelphia, PA, USA.

Carina Lott (C)

Division of Orthopedics, Children's Hospital of Philadelphia, Philadelphia, PA, USA.

Abigail J Clark (AJ)

Division of Orthopedics, Children's Hospital of Philadelphia, Philadelphia, PA, USA.

Frank Mentch (F)

The Center for Applied Genomics, Children's Hospital of Philadelphia, Philadelphia, PA, USA.

Renata Pellegrino Da Silva (RP)

Division of Human Genetics, Children's Hospital of Philadelphia, Philadelphia, PA, USA.
The Center for Applied Genomics, Children's Hospital of Philadelphia, Philadelphia, PA, USA.
Division of Orthopedics, Children's Hospital of Philadelphia, Philadelphia, PA, USA.

Danielle R Rux (DR)

Division of Orthopedics, Children's Hospital of Philadelphia, Philadelphia, PA, USA.

Robert Campbell (R)

Division of Orthopedics, Children's Hospital of Philadelphia, Philadelphia, PA, USA.
Department of Pediatrics, Perelman School of Medicine, Children's Hospital of Philadelphia, University of Pennsylvania, 3615 Civic Center Blvd, Philadelphia, PA, 19104, USA.

Cara Skraban (C)

Division of Human Genetics, Children's Hospital of Philadelphia, Philadelphia, PA, USA.
Department of Pediatrics, Perelman School of Medicine, Children's Hospital of Philadelphia, University of Pennsylvania, 3615 Civic Center Blvd, Philadelphia, PA, 19104, USA.

Xiang Wang (X)

The Center for Applied Genomics, Children's Hospital of Philadelphia, Philadelphia, PA, USA.

Jason B Anari (JB)

Division of Orthopedics, Children's Hospital of Philadelphia, Philadelphia, PA, USA.

Benjamin Sinder (B)

Division of Orthopedics, Children's Hospital of Philadelphia, Philadelphia, PA, USA.

Patrick J Cahill (PJ)

Division of Orthopedics, Children's Hospital of Philadelphia, Philadelphia, PA, USA.
Department of Pediatrics, Perelman School of Medicine, Children's Hospital of Philadelphia, University of Pennsylvania, 3615 Civic Center Blvd, Philadelphia, PA, 19104, USA.

Patrick Sleiman (P)

Division of Human Genetics, Children's Hospital of Philadelphia, Philadelphia, PA, USA.
The Center for Applied Genomics, Children's Hospital of Philadelphia, Philadelphia, PA, USA.
Department of Pediatrics, Perelman School of Medicine, Children's Hospital of Philadelphia, University of Pennsylvania, 3615 Civic Center Blvd, Philadelphia, PA, 19104, USA.

Hakon Hakonarson (H)

Division of Human Genetics, Children's Hospital of Philadelphia, Philadelphia, PA, USA. hakonarson@email.chop.edu.
The Center for Applied Genomics, Children's Hospital of Philadelphia, Philadelphia, PA, USA. hakonarson@email.chop.edu.
Department of Pediatrics, Perelman School of Medicine, Children's Hospital of Philadelphia, University of Pennsylvania, 3615 Civic Center Blvd, Philadelphia, PA, 19104, USA. hakonarson@email.chop.edu.
Endowed Chair in Genomic Research, Division of Pulmonary Medicine, The Joseph Stokes, Jr. Research Institute, Children's Hospital of Philadelphia, Philadelphia, PA, USA. hakonarson@email.chop.edu.

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