Inclusion Bodies: Status Quo and Perspectives.
E. coli
IB-based products
Inclusion bodies
Inclusion body processing
Recombinant protein production, inclusion body refolding, inclusion body challenges, inclusion body quality
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2023
2023
Historique:
entrez:
19
1
2023
pubmed:
20
1
2023
medline:
24
1
2023
Statut:
ppublish
Résumé
Multiple E. coli cultivations, producing recombinant proteins, lead to the formation of inclusion bodies (IBs). IBs historically were considered as nondesired by-products, due to their time- and cost-intensive purification. Nowadays, many obstacles in IB processing can be overcome. As a consequence, several industrial processes with E. coli favor IB formation over soluble production options due to the high space time yields obtained. Within this chapter, we discuss the state-of-the art biopharmaceutical IB process, review its challenges, highlight the recent developments and perspectives, and also propose alternative solutions, compared to the state-of-the art processing.
Identifiants
pubmed: 36656513
doi: 10.1007/978-1-0716-2930-7_1
doi:
Substances chimiques
Recombinant Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1-13Informations de copyright
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
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