Avidity-based bright and photostable light-up aptamers for single-molecule mRNA imaging.
Journal
Nature chemical biology
ISSN: 1552-4469
Titre abrégé: Nat Chem Biol
Pays: United States
ID NLM: 101231976
Informations de publication
Date de publication:
04 2023
04 2023
Historique:
received:
26
01
2022
accepted:
17
11
2022
medline:
31
3
2023
pubmed:
20
1
2023
entrez:
19
1
2023
Statut:
ppublish
Résumé
Fluorescent light-up aptamers (FLAPs) have emerged as valuable tools to visualize RNAs, but are mostly limited by their poor brightness, low photostability, and high fluorescence background in live cells. Exploiting the avidity concept, here we present two of the brightest FLAPs with the strongest aptamer-dye interaction, high fluorogenicity, and remarkable photostability. They consist of dimeric fluorophore-binding aptamers (biRhoBAST and biSiRA) embedded in an RNA scaffold and their bivalent fluorophore ligands (bivalent tetramethylrhodamine TMR
Identifiants
pubmed: 36658339
doi: 10.1038/s41589-022-01228-8
pii: 10.1038/s41589-022-01228-8
doi:
Substances chimiques
RNA, Messenger
0
Aptamers, Nucleotide
0
RNA
63231-63-0
Fluorescent Dyes
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
478-487Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer Nature America, Inc.
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