The non-coding RNA SVALKA locus produces a cis-natural antisense transcript that negatively regulates the expression of CBF1 and biomass production at normal temperatures.
Arabidopsis
CBF1
SVALKA
cis-natural antisense transcript
non-coding transcription
Journal
Plant communications
ISSN: 2590-3462
Titre abrégé: Plant Commun
Pays: China
ID NLM: 101769147
Informations de publication
Date de publication:
10 07 2023
10 07 2023
Historique:
received:
12
12
2022
revised:
17
01
2023
accepted:
18
01
2023
medline:
13
7
2023
pubmed:
23
1
2023
entrez:
22
1
2023
Statut:
ppublish
Résumé
Non-coding transcription is present in all eukaryotic genomes, but we lack fundamental knowledge about its importance for an organism's ability to develop properly. In plants, emerging evidence highlights the essential biological role of non-coding transcription in the regulation of coding transcription. However, we have few molecular insights into this regulation. Here, we show that a long isoform of the long non-coding RNA SVALKA-L (SVK-L) forms a natural antisense transcript to the host gene CBF1 and negatively regulates CBF1 mRNA levels at normal temperatures in the model plant Arabidopsis thaliana. Furthermore, we show detailed evidence for the specific mode of action of SVK-L. This pathway includes the formation of double-stranded RNA that is recognized by the DICER proteins and subsequent downregulation of CBF1 mRNA levels. Thus, the CBF1-SVK regulatory circuit is not only important for its previously known role in cold temperature acclimation but also for biomass production at normal temperatures. Our study characterizes the developmental role of SVK-L and offers mechanistic insight into how biologically important overlapping natural antisense transcripts can act on and fine-tune the steady-state levels of their host gene's mRNA.
Identifiants
pubmed: 36681861
pii: S2590-3462(23)00049-4
doi: 10.1016/j.xplc.2023.100551
pmc: PMC10363475
pii:
doi:
Substances chimiques
RNA, Untranslated
0
RNA, Messenger
0
CBF1 protein, Arabidopsis
0
Trans-Activators
0
Arabidopsis Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
100551Informations de copyright
Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.
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