Generation of nucleotide-linked resins for identification of novel binding proteins.
2′,3′-cyclic nucleotide monophosphate
Binding proteins
Cyclic nucleotide
Nucleotide coupling
Nucleotide resin
Nucleotide signaling
Protein pull-down
Pull-down
Journal
Methods in enzymology
ISSN: 1557-7988
Titre abrégé: Methods Enzymol
Pays: United States
ID NLM: 0212271
Informations de publication
Date de publication:
2023
2023
Historique:
entrez:
22
1
2023
pubmed:
23
1
2023
medline:
25
1
2023
Statut:
ppublish
Résumé
Organisms use numerous nucleotide-containing compounds as intracellular signals to control behavior. Identifying the biomolecules responsible to sensing and responding to changes in signaling molecule concentration is an important area of research. However, identifying the binding proteins can be challenging when there is no prior information available about binding motifs. In this chapter, we describe a straightforward method to generate nucleotide-linked resins for use in pull-down experiments to identify binding proteins. The protocol outlined in this chapter also can be adapted to generate custom resins linked to other molecules of interest.
Identifiants
pubmed: 36682867
pii: S0076-6879(22)00377-9
doi: 10.1016/bs.mie.2022.08.052
pii:
doi:
Substances chimiques
Nucleotides
0
Carrier Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
323-330Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM125842
Pays : United States
Informations de copyright
Copyright © 2023 Elsevier Inc. All rights reserved.