A replication fork determinant for the establishment of sister chromatid cohesion.

Chromatids / metabolism Nuclear Proteins / metabolism Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae Proteins / genetics DNA Replication Cell Cycle Proteins / genetics DNA Acetyltransferases / genetics
DNA replication DNA structures Eco1 chromosome segregation cohesin cohesin acetylation

Journal

Cell
ISSN: 1097-4172
Titre abrégé: Cell
Pays: United States
ID NLM: 0413066

Informations de publication

Date de publication:
16 02 2023
Historique:
received: 14 08 2022
revised: 08 11 2022
accepted: 22 12 2022
pubmed: 25 1 2023
medline: 25 2 2023
entrez: 24 1 2023
Statut: ppublish

Résumé

Concomitant with DNA replication, the chromosomal cohesin complex establishes cohesion between newly replicated sister chromatids. Cohesion establishment requires acetylation of conserved cohesin lysine residues by Eco1 acetyltransferase. Here, we explore how cohesin acetylation is linked to DNA replication. Biochemical reconstitution of replication-coupled cohesin acetylation reveals that transient DNA structures, which form during DNA replication, control the acetylation reaction. As polymerases complete lagging strand replication, strand displacement synthesis produces DNA flaps that are trimmed to result in nicked double-stranded DNA. Both flaps and nicks stimulate cohesin acetylation, while subsequent nick ligation to complete Okazaki fragment maturation terminates the acetylation reaction. A flapped or nicked DNA substrate constitutes a transient molecular clue that directs cohesin acetylation to a window behind the replication fork, next to where cohesin likely entraps both sister chromatids. Our results provide an explanation for how DNA replication is linked to sister chromatid cohesion establishment.

Identifiants

DOI: 10.1016/j.cell.2022.12.044 PMID: 36693376
pubmed: 36693376
pii: S0092-8674(22)01587-2
doi: 10.1016/j.cell.2022.12.044
pii:
doi:

Substances chimiques

Nuclear Proteins 0
Saccharomyces cerevisiae Proteins 0
Cell Cycle Proteins 0
DNA 9007-49-2
Acetyltransferases EC 2.3.1.-
ECO1 protein, S cerevisiae EC 2.3.1.-

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

837-849.e11

Subventions

Organisme : Wellcome Trust
ID : 220244/Z/20/Z
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 219527/Z/19/Z
Pays : United Kingdom

Commentaires et corrections

Type : CommentIn

Informations de copyright

Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests The authors declare no competing interests.

Auteurs

Masashi Minamino (M)

Chromosome Segregation Laboratory, The Francis Crick Institute, London NW1 1AT, UK.

Céline Bouchoux (C)

Chromosome Segregation Laboratory, The Francis Crick Institute, London NW1 1AT, UK.

Berta Canal (B)

Chromosome Replication Laboratory, The Francis Crick Institute, London NW1 1AT, UK.

John F X Diffley (JFX)

Chromosome Replication Laboratory, The Francis Crick Institute, London NW1 1AT, UK.

Frank Uhlmann (F)

Chromosome Segregation Laboratory, The Francis Crick Institute, London NW1 1AT, UK. Electronic address: frank.uhlmann@crick.ac.uk.

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Classifications MeSH

questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Structures de chromosome › Chromatides › A replication fork determinant for the...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines nucléaires › A replication fork determinant for the...
questionsmedicales.fr › Eucaryotes › Champignons › Levures › Saccharomyces › Saccharomyces cerevisiae › A replication fork determinant for the...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines fongiques › Protéines de Saccharomyces cerevisiae › A replication fork determinant for the...
questionsmedicales.fr › Phénomènes génétiques › Réplication de l'ADN › A replication fork determinant for the...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines du cycle cellulaire › A replication fork determinant for the...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › A replication fork determinant for the...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Transferases › Acyltransferases › Acetyltransferases › A replication fork determinant for the...