A novel junctional epithelial cell line, mHAT-JE01, derived from incisor epithelial cells.
Cell line
Dental epithelial stem cell
FDC-SP
Junctional epithelium
ODAM
Journal
Journal of oral biosciences
ISSN: 1880-3865
Titre abrégé: J Oral Biosci
Pays: Netherlands
ID NLM: 101226721
Informations de publication
Date de publication:
03 2023
03 2023
Historique:
received:
09
12
2022
revised:
16
01
2023
accepted:
17
01
2023
pubmed:
25
1
2023
medline:
8
3
2023
entrez:
24
1
2023
Statut:
ppublish
Résumé
Junctional epithelium (JE) connects the tooth surface and gingival epithelium and adheres directly to the tooth enamel. JE plays an important role as a barrier preventing the invasion of exogenous bacteria and substances. However, the cellular characteristics of this epithelium have not been adequately described, because no useful in vitro experimental model exists for JE. We generated a novel JE cell line, mHAT-JE01, using naturally immortalized dental epithelium derived from incisor labial cervical cells and by selecting cells that adhered to apatite. mHAT-JE01 was characterized by immunohistochemistry and quantitative reverse transcription-polymerase chain reaction and compared with the gingival epithelial cell line, mOE-PE01. The mHAT-JE01 cells had a higher capacity for producing JE-specific markers than oral mucous epithelial cells. In addition, the presence of lipopolysaccharides from Porphyromonas gingivalis downregulated the expression of JE protein markers in mHAT-JE01 cells. This cell line is stable and presents the opportunity to characterize JE efficiently, which is essential for the prevention and treatment of periodontal disease.
Identifiants
pubmed: 36693475
pii: S1349-0079(23)00004-X
doi: 10.1016/j.job.2023.01.004
pii:
doi:
Substances chimiques
Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
47-54Informations de copyright
Copyright © 2023 Japanese Association for Oral Biology. Published by Elsevier B.V. All rights reserved.