Molecular phenotyping of single pancreatic islet leader beta cells by "Flash-Seq".

Insulin-Secreting Cells / metabolism Islets of Langerhans / metabolism Insulin Secretion Gene Expression Regulation Cell Line Insulin / metabolism Glucose / metabolism
Calcium signalling connectivity Diabetes Insulin secretion RNA-Seq beta cell

Journal

Life sciences
ISSN: 1879-0631
Titre abrégé: Life Sci
Pays: Netherlands
ID NLM: 0375521

Informations de publication

Date de publication:
01 Mar 2023
Historique:
received: 11 10 2022
revised: 11 01 2023
accepted: 20 01 2023
pubmed: 28 1 2023
medline: 15 2 2023
entrez: 27 1 2023
Statut: ppublish

Résumé

Spatially-organized increases in cytosolic Ca High speed confocal Ca Distinct Ca The existence of both a discrete transcriptome and unique localisation implies a role for these features in defining the specialized function of leaders. These data also raise the possibility that localised signalling between delta and leader cells contributes to the initiation and propagation of islet Ca

Identifiants

DOI: 10.1016/j.lfs.2023.121436 PMID: 36706832
pubmed: 36706832
pii: S0024-3205(23)00070-X
doi: 10.1016/j.lfs.2023.121436
pii:
doi:

Substances chimiques

Insulin 0
Glucose IY9XDZ35W2

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

121436

Subventions

Organisme : Medical Research Council
ID : MR/P023223/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/R022259/1
Pays : United Kingdom

Informations de copyright

Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest GR has received grant funding from, and is a consultant for, Sun Pharmaceuticals Inc. This company was not involved in the present study.

Auteurs

Pauline Chabosseau (P)

Centre de Recherche du CHUM, Faculté de Médicine, Université de Montréal, Montréal, QC, Canada.

Fiona Yong (F)

Section of Cell Biology and Functional Genomics, Division of Diabetes, Endocrinology and Metabolism, Department of Metabolism, Digestion and Reproduction, Faculty of Medicine, Imperial College London, London W12 0NN, United Kingdom; Lee Kong Chian Imperial Medical School, Nanyang Technological University, Singapore.

Luis F Delgadillo-Silva (LF)

Centre de Recherche du CHUM, Faculté de Médicine, Université de Montréal, Montréal, QC, Canada.

Eun Young Lee (EY)

Department of Medicine, Washington University School of Medicine, Saint Louis, MO, United States; Division of Endocrinology and Metabolism, Department of Internal Medicine, Seoul St. Mary's Hospital, The Catholic University of Korea, Seoul, South Korea.

Rana Melhem (R)

Centre de Recherche du CHUM, Faculté de Médicine, Université de Montréal, Montréal, QC, Canada.

Shiying Li (S)

Centre de Recherche du CHUM, Faculté de Médicine, Université de Montréal, Montréal, QC, Canada.

Nidhi Gandhi (N)

Section of Cell Biology and Functional Genomics, Division of Diabetes, Endocrinology and Metabolism, Department of Metabolism, Digestion and Reproduction, Faculty of Medicine, Imperial College London, London W12 0NN, United Kingdom.

Jules Wastin (J)

Centre de Recherche du CHUM, Faculté de Médicine, Université de Montréal, Montréal, QC, Canada.

Livia Lopez Noriega (LL)

Section of Cell Biology and Functional Genomics, Division of Diabetes, Endocrinology and Metabolism, Department of Metabolism, Digestion and Reproduction, Faculty of Medicine, Imperial College London, London W12 0NN, United Kingdom.

Isabelle Leclerc (I)

Centre de Recherche du CHUM, Faculté de Médicine, Université de Montréal, Montréal, QC, Canada.

Yusuf Ali (Y)

Lee Kong Chian Imperial Medical School, Nanyang Technological University, Singapore.

Jing W Hughes (JW)

Department of Medicine, Washington University School of Medicine, Saint Louis, MO, United States.

Robert Sladek (R)

Departments of Medicine and Human Genetics, McGill University and Genome Quebec Innovation Centre, Montreal, QC, Canada.

Aida Martinez-Sanchez (A)

Section of Cell Biology and Functional Genomics, Division of Diabetes, Endocrinology and Metabolism, Department of Metabolism, Digestion and Reproduction, Faculty of Medicine, Imperial College London, London W12 0NN, United Kingdom.

Guy A Rutter (GA)

Centre de Recherche du CHUM, Faculté de Médicine, Université de Montréal, Montréal, QC, Canada; Section of Cell Biology and Functional Genomics, Division of Diabetes, Endocrinology and Metabolism, Department of Metabolism, Digestion and Reproduction, Faculty of Medicine, Imperial College London, London W12 0NN, United Kingdom; Lee Kong Chian Imperial Medical School, Nanyang Technological University, Singapore. Electronic address: guy.rutter@umontreal.ca.

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Classifications MeSH

questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules entéroendocrines › Cellules à insuline › Molecular phenotyping of single pancreatic...
questionsmedicales.fr › Système endocrine › Glandes endocrines › Ilots pancréatiques › Molecular phenotyping of single pancreatic...
questionsmedicales.fr › Phénomènes physiologiques › Sécrétion d'insuline › Molecular phenotyping of single pancreatic...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Molecular phenotyping of single pancreatic...
questionsmedicales.fr › Cellules › Cellules cultivées › Lignée cellulaire › Molecular phenotyping of single pancreatic...
questionsmedicales.fr › Acides aminés, peptides et protéines › Peptides › Hormones peptidiques › Hormones pancréatiques › Insulines › Insuline › Molecular phenotyping of single pancreatic...
questionsmedicales.fr › Glucides › Sucres › Oses › Hexose › Glucose › Molecular phenotyping of single pancreatic...