Iron-sulfur clusters are involved in post-translational arginylation.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
28 01 2023
28 01 2023
Historique:
received:
13
05
2021
accepted:
18
01
2023
pubmed:
29
1
2023
medline:
1
2
2023
entrez:
28
1
2023
Statut:
epublish
Résumé
Eukaryotic arginylation is an essential post-translational modification that modulates protein stability and regulates protein half-life. Arginylation is catalyzed by a family of enzymes known as the arginyl-tRNA transferases (ATE1s), which are conserved across the eukaryotic domain. Despite their conservation and importance, little is known regarding the structure, mechanism, and regulation of ATE1s. In this work, we show that ATE1s bind a previously undiscovered [Fe-S] cluster that is conserved across evolution. We characterize the nature of this [Fe-S] cluster and find that the presence of the [Fe-S] cluster in ATE1 is linked to its arginylation activity, both in vitro and in vivo, and the initiation of the yeast stress response. Importantly, the ATE1 [Fe-S] cluster is oxygen-sensitive, which could be a molecular mechanism of the N-degron pathway to sense oxidative stress. Taken together, our data provide the framework of a cluster-based paradigm of ATE1 regulatory control.
Identifiants
pubmed: 36709327
doi: 10.1038/s41467-023-36158-z
pii: 10.1038/s41467-023-36158-z
pmc: PMC9884297
doi:
Substances chimiques
Aminoacyltransferases
EC 2.3.2.-
Proteins
0
Iron-Sulfur Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
458Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM133497
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM138557
Pays : United States
Organisme : NIGMS NIH HHS
ID : T34 GM136497
Pays : United States
Organisme : NIGMS NIH HHS
ID : P41 GM103393
Pays : United States
Informations de copyright
© 2023. The Author(s).
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