The increased efficiency of porphyran hydrolysis by constructing a multifunctional enzyme complex from marine microorganisms.
Cellulosome
Marine biomass
Porphyran
Porphyranase
Porphyranase complex
Journal
Enzyme and microbial technology
ISSN: 1879-0909
Titre abrégé: Enzyme Microb Technol
Pays: United States
ID NLM: 8003761
Informations de publication
Date de publication:
Apr 2023
Apr 2023
Historique:
received:
03
11
2022
revised:
23
12
2022
accepted:
24
01
2023
pubmed:
30
1
2023
medline:
7
3
2023
entrez:
29
1
2023
Statut:
ppublish
Résumé
Porphyran, a polysaccharide composed of red algae, is a source of a multifunctional oligosaccharide material and raw biomass with various physiological activities. The glycolysis of porphyrans into oligosaccharides through various porphyranases is an approach for obtaining high-quality and promising alternative resources. In this study, porphyran was extracted from Porphyra yezoensis and used as a research substrate. We also established an efficient hydrolysis method using an enzymatic complex obtained through cohesin-dockerin interactions that degrade natural polysaccharides. The cohesion-dockerin interaction is designed to genetically bind the dockerin module to the end of an existing enzyme and then attach the cohesin module to obtain a protein complex. The designed protein complex has been shown to further increase the activity on the substrate, which can be considered a useful method to obtain efficient oligosaccharides or monosaccharides through hydrolysis of red algae for bioresources.
Identifiants
pubmed: 36709516
pii: S0141-0229(23)00015-7
doi: 10.1016/j.enzmictec.2023.110207
pii:
doi:
Substances chimiques
Multifunctional Enzymes
0
porphyran
11016-36-7
Multienzyme Complexes
0
Sepharose
9012-36-6
Bacterial Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
110207Informations de copyright
Copyright © 2023 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.