Membrane Targeted Azobenzene Drives Optical Modulation of Bacterial Membrane Potential.
bacterial cell electrophysiology
bacterial electrical signaling
bioelectricity
nanomaterials
optostimulation
photonics
Journal
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
ISSN: 2198-3844
Titre abrégé: Adv Sci (Weinh)
Pays: Germany
ID NLM: 101664569
Informations de publication
Date de publication:
03 2023
03 2023
Historique:
revised:
17
01
2023
received:
06
09
2022
pubmed:
30
1
2023
medline:
21
3
2023
entrez:
29
1
2023
Statut:
ppublish
Résumé
Recent studies have shown that bacterial membrane potential is dynamic and plays signaling roles. Yet, little is still known about the mechanisms of membrane potential dynamics regulation-owing to a scarcity of appropriate research tools. Optical modulation of bacterial membrane potential could fill this gap and provide a new approach for studying and controlling bacterial physiology and electrical signaling. Here, the authors show that a membrane-targeted azobenzene (Ziapin2) can be used to photo-modulate the membrane potential in cells of the Gram-positive bacterium Bacillus subtilis. It is found that upon exposure to blue-green light (λ = 470 nm), isomerization of Ziapin2 in the bacteria membrane induces hyperpolarization of the potential. To investigate the origin of this phenomenon, ion-channel-deletion strains and ion channel blockers are examined. The authors found that in presence of the chloride channel blocker idanyloxyacetic acid-94 (IAA-94) or in absence of KtrAB potassium transporter, the hyperpolarization response is attenuated. These results reveal that the Ziapin2 isomerization can induce ion channel opening in the bacterial membrane and suggest that Ziapin2 can be used for studying and controlling bacterial electrical signaling. This new optical tool could contribute to better understand various microbial phenomena, such as biofilm electric signaling and antimicrobial resistance.
Identifiants
pubmed: 36710255
doi: 10.1002/advs.202205007
pmc: PMC10015841
doi:
Substances chimiques
azobenzene
F0U1H6UG5C
Azo Compounds
0
Potassium
RWP5GA015D
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2205007Subventions
Organisme : Fondazione Cariplo
ID : 2018-0979
Organisme : Engineering and Physical Sciences Research Council
Organisme : Warwick Integrative Synthetic Biology Centre
ID : BB/M017982/1
Organisme : MRC Doctoral Training Partnership
ID : MR/N014294/1
Organisme : Biotechnology and Biological Sciences Research Council
Pays : United Kingdom
Informations de copyright
© 2023 The Authors. Advanced Science published by Wiley-VCH GmbH.
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