ABCB-mediated shootward auxin transport feeds into the root clock.
ABCB
auxin transport
lateral root
root meristem
Journal
EMBO reports
ISSN: 1469-3178
Titre abrégé: EMBO Rep
Pays: England
ID NLM: 100963049
Informations de publication
Date de publication:
05 04 2023
05 04 2023
Historique:
revised:
29
11
2022
received:
11
10
2022
accepted:
10
01
2023
pmc-release:
31
01
2024
medline:
6
4
2023
pubmed:
1
2
2023
entrez:
31
1
2023
Statut:
ppublish
Résumé
Although strongly influenced by environmental conditions, lateral root (LR) positioning along the primary root appears to follow obediently an internal spacing mechanism dictated by auxin oscillations that prepattern the primary root, referred to as the root clock. Surprisingly, none of the hitherto characterized PIN- and ABCB-type auxin transporters seem to be involved in this LR prepatterning mechanism. Here, we characterize ABCB15, 16, 17, 18, and 22 (ABCB15-22) as novel auxin-transporting ABCBs. Knock-down and genome editing of this genetically linked group of ABCBs caused strongly reduced LR densities. These phenotypes were correlated with reduced amplitude, but not reduced frequency of the root clock oscillation. High-resolution auxin transport assays and tissue-specific silencing revealed contributions of ABCB15-22 to shootward auxin transport in the lateral root cap (LRC) and epidermis, thereby explaining the reduced auxin oscillation. Jointly, these data support a model in which LRC-derived auxin contributes to the root clock amplitude.
Identifiants
pubmed: 36718777
doi: 10.15252/embr.202256271
pmc: PMC10074126
doi:
Substances chimiques
Arabidopsis Proteins
0
Membrane Transport Proteins
0
Indoleacetic Acids
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e56271Subventions
Organisme : China Scholarship Council (CSC)
Organisme : EC ¦ European Research Council (ERC)
ID : 757683
Organisme : Fonds Wetenschappelijk Onderzoek (FWO)
ID : G002220N
Organisme : PBC
Organisme : Swiss National Funds
ID : 31003A-165877/1
Informations de copyright
© 2023 The Authors.
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