Flies (Insecta, Diptera) collected in the environment of dairy farms as carriers of Rotavirus A and betacoronavirus.
BCoV
RVA
Vector-borne diseases
dairy cows
flying insects
Journal
Journal of applied microbiology
ISSN: 1365-2672
Titre abrégé: J Appl Microbiol
Pays: England
ID NLM: 9706280
Informations de publication
Date de publication:
01 Mar 2023
01 Mar 2023
Historique:
received:
08
12
2022
revised:
24
01
2023
accepted:
31
01
2023
pubmed:
2
2
2023
medline:
4
3
2023
entrez:
1
2
2023
Statut:
ppublish
Résumé
We aimed to investigate the prevalence of rotavirus and coronavirus in dipterans that commonly inhabit the environment of dairy farms. We collected 217 insect specimens from nine dairy farms, which were examined through hemi-nested RT-PCR followed by Sanger sequencing in search of VP1 and N genes for rotavirus and bovine coronavirus-BCoV, respectively. With a predominance of Muscidae (152/217 = 70%) 11 families of Diptera were identified. Rotavirus A (RVA) and betacoronavirus (BCoV) were detected in 14.7% (32/217) and 4.6% (10/217) of the dipterans, respectively. Sequencing of the amplicons was possible for 11.5% (25/217) of RVA and 0.5% (1/217) of BCoV, confirming the presence of these pathogens. Our findings highlight the role of dipterans as carriers of RVA and BCoV of great relevance for public and animal health.
Identifiants
pubmed: 36725209
pii: 7022323
doi: 10.1093/jambio/lxad020
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : FAPESP
ID : 2015/19688-8
Informations de copyright
© The Author(s) 2023. Published by Oxford University Press on behalf of Applied Microbiology International.