Flexibility of the Rotavirus NSP2 C-Terminal Region Supports Factory Formation via Liquid-Liquid Phase Separation.
LLPS
NSP2
NSP5
rotavirus
viral factory
viroplasm
Journal
Journal of virology
ISSN: 1098-5514
Titre abrégé: J Virol
Pays: United States
ID NLM: 0113724
Informations de publication
Date de publication:
28 02 2023
28 02 2023
Historique:
pubmed:
8
2
2023
medline:
3
3
2023
entrez:
7
2
2023
Statut:
ppublish
Résumé
Many viruses sequester the materials needed for their replication into discrete subcellular factories. For rotaviruses (RVs), these factories are called viroplasms, and they are formed in the host cell cytosol via the process of liquid-liquid phase separation (LLPS). The nonstructural protein 2 (NSP2) and its binding partner, nonstructural protein 5 (NSP5), are critical for viroplasm biogenesis. Yet it is not fully understood how NSP2 and NSP5 cooperate to form factories. The C-terminal region (CTR) of NSP2 (residues 291 to 317) is flexible, allowing it to participate in domain-swapping interactions that promote interoctamer interactions and, presumably, viroplasm formation. Molecular dynamics simulations showed that a lysine-to-glutamic acid change at position 294 (K294E) reduces NSP2 CTR flexibility
Identifiants
pubmed: 36749077
doi: 10.1128/jvi.00039-23
pmc: PMC9973012
mid: EMS170586
doi:
Substances chimiques
Viral Nonstructural Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0003923Subventions
Organisme : NIAID NIH HHS
ID : R21 AI152439
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI116815
Pays : United States
Organisme : Wellcome Trust
ID : 213437
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 213437/Z/18/Z
Pays : United Kingdom
Organisme : Wellcome Trust
Pays : United Kingdom
Références
J Virol. 2019 Dec 12;94(1):
pubmed: 31619556
Front Physiol. 2022 Mar 23;13:836870
pubmed: 35492603
IEEE Trans Image Process. 1995;4(3):370-8
pubmed: 18289986
J Mol Biol. 2019 Aug 9;431(17):3124-3138
pubmed: 31233764
J Virol. 2002 Apr;76(7):3461-70
pubmed: 11884570
J Biol Chem. 2004 Mar 12;279(11):10624-33
pubmed: 14699117
Cell Rep. 2020 Sep 29;32(13):108205
pubmed: 32997981
Sci Rep. 2017 Aug 24;7(1):9301
pubmed: 28839154
Virology. 2015 Mar;477:32-41
pubmed: 25635339
J Virol. 2012 Oct;86(19):10547-57
pubmed: 22811529
Curr Opin Virol. 2016 Aug;19:11-5
pubmed: 27341619
Adv Virus Res. 2000;55:185-229
pubmed: 11050943
Viruses. 2021 Feb 25;13(3):
pubmed: 33669141
J Gen Virol. 1996 Sep;77 ( Pt 9):2059-65
pubmed: 8811003
J Virol. 2002 Jul;76(14):7082-93
pubmed: 12072508
J Gen Virol. 2013 Jul;94(Pt 7):1576-1586
pubmed: 23515026
Virology. 1989 Oct;172(2):616-27
pubmed: 2552662
Curr Protoc Microbiol. 2009 Nov;Chapter 15:Unit 15C.3
pubmed: 19885940
J Gen Virol. 2004 Mar;85(Pt 3):625-634
pubmed: 14993647
J Virol. 2020 Jul 16;94(15):
pubmed: 32461314
Viruses. 2021 Jul 12;13(7):
pubmed: 34372555
PLoS One. 2012;7(10):e47947
pubmed: 23110139
Proc Natl Acad Sci U S A. 2018 Dec 18;115(51):E12015-E12023
pubmed: 30509975
EMBO J. 2011 Jan 19;30(2):408-16
pubmed: 21157433
J Comput Chem. 2004 Oct;25(13):1605-12
pubmed: 15264254
J Virol. 2014 Jan;88(2):786-98
pubmed: 24198401
Nat Commun. 2019 May 17;10(1):2216
pubmed: 31101900
PLoS Pathog. 2021 Oct 14;17(10):e1009926
pubmed: 34648608
J Virol. 2010 Jul;84(13):6782-98
pubmed: 20335253
Cell Microbiol. 2013 Jan;15(1):24-34
pubmed: 22978691
J Mol Biol. 2011 Oct 14;413(1):209-21
pubmed: 21864538
Proc Natl Acad Sci U S A. 2004 Nov 16;101(46):16304-9
pubmed: 15520389
Nat Methods. 2012 Jun 28;9(7):676-82
pubmed: 22743772
J Virol. 2006 Nov;80(21):10829-35
pubmed: 16928740
Elife. 2017 Sep 18;6:
pubmed: 28922109
J Gen Virol. 2010 Jul;91(Pt 7):1782-93
pubmed: 20200190
Nat Rev Microbiol. 2012 Jan 23;10(3):165-77
pubmed: 22266782
Proc Natl Acad Sci U S A. 2017 Feb 28;114(9):2349-2354
pubmed: 28137864
J Virol. 2001 May;75(10):4519-27
pubmed: 11312322
EMBO J. 2021 Nov 2;40(21):e107711
pubmed: 34524703
J Virol. 2017 Mar 13;91(7):
pubmed: 28100623
Nature. 2002 May 16;417(6886):311-5
pubmed: 12015608
Nat Methods. 2022 Jul;19(7):829-832
pubmed: 35654950
Curr Top Microbiol Immunol. 2006;309:169-87
pubmed: 16909900
Virus Res. 2021 Sep;302:198488
pubmed: 34146610
Proteins. 2010 Jun;78(8):1950-8
pubmed: 20408171
Proc Natl Acad Sci U S A. 2021 Oct 12;118(41):
pubmed: 34615715
J Gen Virol. 1999 Feb;80 ( Pt 2):333-339
pubmed: 10073692
J Virol. 2019 Nov 26;93(24):
pubmed: 31597761
Front Immunol. 2022 Aug 09;13:985622
pubmed: 36016945