The hydroxyproline O-arabinosyltransferase FIN4 is required for tomato pollen intine development.


Journal

Plant reproduction
ISSN: 2194-7961
Titre abrégé: Plant Reprod
Pays: Germany
ID NLM: 101602701

Informations de publication

Date de publication:
Jun 2023
Historique:
received: 03 11 2022
accepted: 20 01 2023
medline: 12 6 2023
pubmed: 8 2 2023
entrez: 7 2 2023
Statut: ppublish

Résumé

The pollen grain cell wall is a highly specialized structure composed of distinct layers formed through complex developmental pathways. The production of the innermost intine layer, composed of cellulose, pectin and other polymers, is particularly poorly understood. Here we demonstrate an important and specific role for the hydroxyproline O-arabinosyltransferase (HPAT) FIN4 in tomato intine development. HPATs are plant-specific enzymes which initiate glycosylation of certain cell wall structural proteins and signaling peptides. FIN4 was expressed throughout pollen development in both the developing pollen and surrounding tapetal cells. A fin4 mutant with a partial deletion of the catalytic domain displayed significantly reduced male fertility in vivo and compromised pollen hydration and germination in vitro. However, fin4 pollen that successfully germinated formed morphologically normal pollen tubes with the same growth rate as the wild-type pollen. When we examined mature fin4 pollen, we found they were cytologically normal, and formed morphologically normal exine, but produced significantly thinner intine. During intine deposition at the late stages of pollen development we found fin4 pollen had altered polymer deposition, including reduced cellulose and increased detection of pectin, specifically homogalacturonan with both low and high degrees of methylesterification. Therefore, FIN4 plays an important role in intine formation and, in turn pollen hydration and germination and the process of intine formation involves dynamic changes in the developing pollen cell wall.

Identifiants

pubmed: 36749417
doi: 10.1007/s00497-023-00459-6
pii: 10.1007/s00497-023-00459-6
doi:

Substances chimiques

arabinosyltransferase EC 2.4.2.-
Hydroxyproline RMB44WO89X
Pectins 89NA02M4RX

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

173-191

Subventions

Organisme : Division of Integrative Organismal Systems
ID : IOS-1755482

Informations de copyright

© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

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Auteurs

Syeda Roop Fatima Jaffri (SRF)

Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI, USA.

Holly Scheer (H)

Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI, USA.

Cora A MacAlister (CA)

Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI, USA. coramacalister@gmail.com.

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