Clathrin-Mediated Albumin Clearance in Alveolar Epithelial Cells of Murine Precision-Cut Lung Slices.
acute respiratory distress syndrome
albumin
alveolar epithelium
endocytosis
precision-cut lung slices
protein transport
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
31 Jan 2023
31 Jan 2023
Historique:
received:
26
12
2022
revised:
19
01
2023
accepted:
23
01
2023
entrez:
11
2
2023
pubmed:
12
2
2023
medline:
15
2
2023
Statut:
epublish
Résumé
A hallmark of acute respiratory distress syndrome (ARDS) is an accumulation of protein-rich alveolar edema that impairs gas exchange and leads to worse outcomes. Thus, understanding the mechanisms of alveolar albumin clearance is of high clinical relevance. Here, we investigated the mechanisms of the cellular albumin uptake in a three-dimensional culture of precision-cut lung slices (PCLS). We found that up to 60% of PCLS cells incorporated labeled albumin in a time- and concentration-dependent manner, whereas virtually no uptake of labeled dextran was observed. Of note, at a low temperature (4 °C), saturating albumin receptors with unlabeled albumin and an inhibition of clathrin-mediated endocytosis markedly decreased the endocytic uptake of the labeled protein, implicating a receptor-driven internalization process. Importantly, uptake rates of albumin were comparable in alveolar epithelial type I (ATI) and type II (ATII) cells, as assessed in PCLS from a SftpcCre
Identifiants
pubmed: 36768968
pii: ijms24032644
doi: 10.3390/ijms24032644
pmc: PMC9916738
pii:
doi:
Substances chimiques
Epithelial Cell Adhesion Molecule
0
Clathrin
0
Serum Albumin
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Deutsche Forschungsgemeinschaft
ID : DFG/KFO309, P5
Organisme : Deutsche Forschungsgemeinschaft
ID : EXC 2026; Project ID: 390649896
Organisme : Federal Ministry of Education and Research
ID : DZL/ALI 1.5 and 3.4
Organisme : Von Behring-Röntgen-Stiftung
ID : Project 66-LV07
Références
Am J Respir Cell Mol Biol. 2017 Nov;57(5):589-602
pubmed: 28678521
Crit Care. 2020 Aug 28;24(1):529
pubmed: 32859264
Eur Respir J. 2021 Nov 4;58(5):
pubmed: 33863742
Nat Rev Mol Cell Biol. 2009 Sep;10(9):597-608
pubmed: 19696797
Drug Metab Pharmacokinet. 2012;27(3):336-43
pubmed: 22214936
Pharm Res. 2008 Apr;25(4):913-22
pubmed: 17851738
EMBO J. 2020 Nov 2;39(21):e103476
pubmed: 32985719
Am J Respir Cell Mol Biol. 2017 Jul;57(1):18-27
pubmed: 28326803
Int J Nanomedicine. 2019 May 21;14:3723-3741
pubmed: 31190821
Respir Res. 2019 Jul 19;20(1):162
pubmed: 31324219
Annu Rev Microbiol. 2021 Oct 8;75:19-47
pubmed: 33492978
Nat Rev Mol Cell Biol. 2004 Feb;5(2):121-32
pubmed: 15040445
Eur Respir J. 2020 Sep 24;56(3):
pubmed: 32747391
J Physiol. 2004 Oct 1;560(Pt 1):267-80
pubmed: 15297572
Am J Med. 1979 Jul;67(1):32-8
pubmed: 463915
BMC Res Notes. 2017 Mar 9;10(1):121
pubmed: 28274266
Mol Microbiol. 2022 Mar;117(3):578-588
pubmed: 34570407
Science. 2010 Jun 25;328(5986):1662-8
pubmed: 20576885
Biochim Biophys Acta. 2013 Dec;1830(12):5526-34
pubmed: 23639804
Drug Metab Pharmacokinet. 2011;26(5):474-85
pubmed: 21712611
Clin Chest Med. 2000 Sep;21(3):477-90
pubmed: 11019721
J Physiol. 2012 Oct 15;590(20):5167-81
pubmed: 22826129
Am Rev Respir Dis. 1977 Oct;116(4):589-615
pubmed: 921049
Am J Physiol Lung Cell Mol Physiol. 2021 Oct 1;321(4):L734-L735
pubmed: 34549594
Int J Mol Sci. 2021 Sep 18;22(18):
pubmed: 34576249
J Cell Sci. 2019 Oct 1;133(5):
pubmed: 31444284
Nat Commun. 2019 Jul 5;10(1):2987
pubmed: 31278260
Am J Physiol Lung Cell Mol Physiol. 2013 Nov 15;305(10):L665-81
pubmed: 24039257
Am J Physiol Lung Cell Mol Physiol. 2007 Oct;293(4):L823-42
pubmed: 17644753
Am J Physiol Lung Cell Mol Physiol. 2019 May 1;316(5):L738-L739
pubmed: 30840484
Am J Physiol Lung Cell Mol Physiol. 2003 Jan;284(1):L187-96
pubmed: 12471015
Am J Physiol Lung Cell Mol Physiol. 2017 Nov 1;313(5):L807-L824
pubmed: 28705909
Am J Physiol Lung Cell Mol Physiol. 2006 May;290(5):L946-55
pubmed: 16361359
N Engl J Med. 2017 Aug 10;377(6):562-572
pubmed: 28792873
Nat Rev Mol Cell Biol. 2002 Apr;3(4):256-66
pubmed: 11994745
Am J Physiol Lung Cell Mol Physiol. 2006 May;290(5):L806-8
pubmed: 16603594
Am J Physiol Lung Cell Mol Physiol. 2021 Jun 1;320(6):L1186-L1193
pubmed: 33689516
PLoS One. 2013 Nov 27;8(11):e81903
pubmed: 24312378
Physiol Rev. 2003 Jul;83(3):871-932
pubmed: 12843411
Biochim Biophys Acta. 2012 Mar;1820(3):264-81
pubmed: 21968002
Proc Am Thorac Soc. 2005;2(3):206-13
pubmed: 16222039
Nat Rev Dis Primers. 2019 Mar 14;5(1):18
pubmed: 30872586
Am J Physiol Lung Cell Mol Physiol. 2003 Feb;284(2):L247-59
pubmed: 12533309
Int J Mol Sci. 2021 Aug 05;22(16):
pubmed: 34445120
Am J Respir Cell Mol Biol. 2020 Jun;62(6):681-691
pubmed: 31991090
Am J Physiol Lung Cell Mol Physiol. 2004 Apr;286(4):L679-89
pubmed: 15003932
J Microencapsul. 1994 May-Jun;11(3):261-9
pubmed: 8064550
Am J Physiol Lung Cell Mol Physiol. 2019 May 1;316(5):L740-L750
pubmed: 30702342