Multicopy suppressor screens reveal convergent evolution of single-gene lysis proteins.

Bacteriophages / genetics Pseudomonas aeruginosa / virology Biological Evolution Genes, Viral

Journal

Nature chemical biology
ISSN: 1552-4469
Titre abrégé: Nat Chem Biol
Pays: United States
ID NLM: 101231976

Informations de publication

Date de publication:
06 2023
Historique:
received: 18 09 2022
accepted: 18 01 2023
medline: 1 6 2023
pubmed: 23 2 2023
entrez: 22 2 2023
Statut: ppublish

Résumé

Single-strand RNA (ssRNA) Fiersviridae phages cause host lysis with a product of single gene (sgl for single-gene lysis; product Sgl) that induces autolysis. Many different Sgls have been discovered, but the molecular targets of only a few have been identified. In this study, we used a high-throughput genetic screen to uncover genome-wide host suppressors of diverse Sgls. In addition to validating known molecular mechanisms, we discovered that the Sgl of PP7, an ssRNA phage of Pseudomonas aeruginosa, targets MurJ, the flippase responsible for lipid II export, previously shown to be the target of the Sgl of coliphage M. These two Sgls, which are unrelated and predicted to have opposite membrane topology, thus represent a case of convergent evolution. We extended the genetic screens to other uncharacterized Sgls and uncovered a common set of multicopy suppressors, suggesting that these Sgls act by the same or similar mechanism.

Identifiants

DOI: 10.1038/s41589-023-01269-7 PMID: 36805702 PMC: PMC10229422
pubmed: 36805702
doi: 10.1038/s41589-023-01269-7
pii: 10.1038/s41589-023-01269-7
pmc: PMC10229422
doi:

Banques de données

figshare
['10.6084/m9.figshare.21714296.v2']

Types de publication

Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

759-766

Subventions

Organisme : NIGMS NIH HHS
ID : R35 GM136396
Pays : United States
Organisme : NCRR NIH HHS
ID : S10 RR029668
Pays : United States
Organisme : NCRR NIH HHS
ID : S10 RR027303
Pays : United States

Informations de copyright

© 2023. The Author(s).

Références

Nucleic Acids Res. 2021 Jan 8;49(D1):D751-D763
pubmed: 33119741
Microb Drug Resist. 2007 Fall;13(3):157-65
pubmed: 17949301
mBio. 2015 May 12;6(3):e00306-15
pubmed: 25968644
J Bacteriol. 2007 Jun;189(11):3987-95
pubmed: 17384195
Nat Commun. 2020 Nov 26;11(1):6009
pubmed: 33243984
Proc Natl Acad Sci U S A. 2011 Jan 25;108(4):1484-9
pubmed: 21173244
Nat Struct Mol Biol. 2017 Feb;24(2):171-176
pubmed: 28024149
Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):25900-25908
pubmed: 31772013
PLoS Biol. 2020 Oct 13;18(10):e3000877
pubmed: 33048924
Nature. 1982 Jan 7;295(5844):35-41
pubmed: 7066091
Curr Opin Biotechnol. 2021 Apr;68:1-7
pubmed: 33007632
Science. 2001 Jun 22;292(5525):2326-9
pubmed: 11423662
Ann N Y Acad Sci. 2013 Jan;1277:54-75
pubmed: 23163477
Sci Adv. 2020 Feb 07;6(6):eaay5981
pubmed: 32083183
Nature. 2016 Dec 22;540(7634):539-543
pubmed: 27880757
PLoS One. 2011 Mar 18;6(3):e17624
pubmed: 21445265
Adv Virus Res. 2019;103:33-70
pubmed: 30635077
J Biol Chem. 2001 Mar 2;276(9):6093-7
pubmed: 11078734
Biotechniques. 1990 May;8(5):528-35
pubmed: 2357375
Nucleic Acids Res. 2021 Jan 8;49(D1):D764-D775
pubmed: 33137183
Nat Commun. 2019 Jan 18;10(1):308
pubmed: 30659179
Mol Syst Biol. 2006;2:2006.0008
pubmed: 16738554
DNA Res. 2005;12(5):291-9
pubmed: 16769691
EMBO J. 2004 Nov 10;23(22):4434-42
pubmed: 15496982
J Bacteriol. 2017 May 25;199(12):
pubmed: 28396351
Nature. 1976 Apr 8;260(5551):500-7
pubmed: 1264203
J Biol Chem. 2019 Mar 8;294(10):3350-3358
pubmed: 30420429
Curr Opin Microbiol. 2020 Aug;56:109-117
pubmed: 33075663
PLoS Biol. 2016 Mar 24;14(3):e1002409
pubmed: 27010970
Int J Mol Sci. 2019 Jan 16;20(2):
pubmed: 30654491
Structure. 2022 Aug 4;30(8):1088-1097.e3
pubmed: 35660157
Crit Rev Biochem Mol Biol. 2017 Oct;52(5):503-542
pubmed: 28644060
Nat Microbiol. 2017 Nov;2(11):1480-1484
pubmed: 28894177
Cell. 1979 Oct;18(2):257-66
pubmed: 387256
J Bacteriol. 2010 Jul;192(13):3243-5
pubmed: 20418394
Microbiology (Reading). 2017 Jul;163(7):961-969
pubmed: 28691656
Nat Rev Microbiol. 2011 Dec 28;10(2):123-36
pubmed: 22203377
Proc Natl Acad Sci U S A. 2015 May 19;112(20):6437-42
pubmed: 25918422
Nucleic Acids Res. 2004 Mar 19;32(5):1792-7
pubmed: 15034147

Auteurs

Benjamin A Adler (BA)

The UC Berkeley-UCSF Graduate Program in Bioengineering, Berkeley, CA, USA.
Department of Bioengineering, University of California, Berkeley, Berkeley, CA, USA.
Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA, USA.

Karthik Chamakura (K)

Department of Biochemistry and Biophysics, Center for Phage Technology, Texas A&M University, College Station, TX, USA.
Armata Pharmaceuticals, Inc., Marina Del Rey, CA, USA.
ORCID: 0000-0002-6196-8571

Heloise Carion (H)

Department of Bioengineering, University of California, Berkeley, Berkeley, CA, USA.

Jonathan Krog (J)

Department of Bioengineering, University of California, Berkeley, Berkeley, CA, USA.

Adam M Deutschbauer (AM)

Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
ORCID: 0000-0003-2728-7622

Ry Young (R)

Department of Biochemistry and Biophysics, Center for Phage Technology, Texas A&M University, College Station, TX, USA.

Vivek K Mutalik (VK)

Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA, USA. vkmutalik@lbl.gov.
Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA. vkmutalik@lbl.gov.
ORCID: 0000-0001-7934-0400

Adam P Arkin (AP)

Department of Bioengineering, University of California, Berkeley, Berkeley, CA, USA. aparkin@lbl.gov.
Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA, USA. aparkin@lbl.gov.
Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA. aparkin@lbl.gov.
ORCID: 0000-0002-4999-2931

Articles similaires

A scenario for an evolutionary selection of ageing.

Tristan Roget, Claire Macmurray, Pierre Jolivet et al.
1.00
Aging Selection, Genetic Biological Evolution Animals Fertility

Complete genome sequences of two novel Ralstonia jumbo phages isolated from leaf litter compost.

Ryota Sasaki, Shuhei Miyashita, Hideki Takahashi
1.00
Genome, Viral Ralstonia Composting Solanum lycopersicum Bacteriophages

The historical transformation of individual concepts into populational ones: an explanatory shift in the gestation of the modern synthesis.

Tiago Rama
1.00
Biological Evolution History, 20th Century Selection, Genetic History, 19th Century Biology

Prediction of strain level phage-host interactions across the Escherichia genus using only genomic information.

Baptiste Gaborieau, Hugo Vaysset, Florian Tesson et al.
1.00
Host Specificity Bacteriophages Genomics Algorithms Escherichia coli

Classifications MeSH

questionsmedicales.fr › Virus › Bactériophages › Multicopy suppressor screens reveal convergent...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Pseudomonadaceae › Pseudomonas › Pseudomonas aeruginosa › Multicopy suppressor screens reveal convergent...
questionsmedicales.fr › Phénomènes biologiques › Évolution biologique › Multicopy suppressor screens reveal convergent...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Génome microbien › Génome viral › Gènes viraux › Multicopy suppressor screens reveal convergent...