Approaches in Hydroxytyrosol Supplementation on Epithelial-Mesenchymal Transition in TGFβ1-Induced Human Respiratory Epithelial Cells.
Humans
Cadherins
/ metabolism
Dietary Supplements
Epithelial-Mesenchymal Transition
/ drug effects
Molecular Docking Simulation
Proto-Oncogene Proteins c-akt
/ metabolism
Transforming Growth Factor beta1
/ metabolism
Vimentin
/ metabolism
Phenylethyl Alcohol
/ pharmacology
Alveolar Epithelial Cells
/ drug effects
EMT
fibrosis
inflammation
olive
rhinosinusitis
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
16 Feb 2023
16 Feb 2023
Historique:
received:
10
12
2022
revised:
03
02
2023
accepted:
06
02
2023
entrez:
25
2
2023
pubmed:
26
2
2023
medline:
3
3
2023
Statut:
epublish
Résumé
Hydroxytyrosol (HT) is an olive polyphenol with anti-inflammatory and antioxidant properties. This study aimed to investigate the effect of HT treatment on epithelial-mesenchymal transition (EMT) in primary human respiratory epithelial cells (RECs) isolated from human nasal turbinate. HT dose-response study and growth kinetic study on RECs was performed. Several approaches on HT treatment and TGFβ1 induction with varying durations and methods was studied. RECs morphology and migration ability were evaluated. Vimentin and E-cadherin immunofluorescence staining and Western blotting [E-cadherin, vimentin, SNAIL/SLUG, AKT, phosphorylated (p)AKT, SMAD2/3 and pSMAD2/3] were performed after 72-h treatment. In silico analysis (molecular docking) of HT was performed to evaluate the potential of HT to bind with the TGFβ receptor. The viability of the HT-treated RECs was concentration-dependent, where the median effective concentration (EC
Identifiants
pubmed: 36835384
pii: ijms24043974
doi: 10.3390/ijms24043974
pmc: PMC9967984
pii:
doi:
Substances chimiques
3,4-dihydroxyphenylethanol
10597-60-1
Cadherins
0
Proto-Oncogene Proteins c-akt
EC 2.7.11.1
Transforming Growth Factor beta1
0
Vimentin
0
Phenylethyl Alcohol
ML9LGA7468
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Universiti Kebangsaan Malaysia
ID : FF-2017-020, FF-2017-363 and FF-2017-273
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