mRNA targeting eliminates the need for the signal recognition particle during membrane protein insertion in bacteria.
Membrane Proteins
/ genetics
Signal Recognition Particle
/ genetics
Escherichia coli Proteins
/ metabolism
Bacterial Proteins
/ metabolism
Escherichia coli
/ genetics
RNA, Messenger
/ genetics
Bacteria
/ metabolism
SEC Translocation Channels
/ genetics
Protein Transport
Ribosomes
/ metabolism
Membrane Transport Proteins
/ metabolism
(p)ppGpp
CP: Microbiology
FtsY
SecYEG translocon
YidC
alarmones
mRNA targeting
signal recognition particle
small membrane proteins
stringent response
translation
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
28 03 2023
28 03 2023
Historique:
received:
13
04
2022
revised:
10
01
2023
accepted:
02
02
2023
medline:
3
4
2023
pubmed:
27
2
2023
entrez:
26
2
2023
Statut:
ppublish
Résumé
Signal-sequence-dependent protein targeting is essential for the spatiotemporal organization of eukaryotic and prokaryotic cells and is facilitated by dedicated protein targeting factors such as the signal recognition particle (SRP). However, targeting signals are not exclusively contained within proteins but can also be present within mRNAs. By in vivo and in vitro assays, we show that mRNA targeting is controlled by the nucleotide content and by secondary structures within mRNAs. mRNA binding to bacterial membranes occurs independently of soluble targeting factors but is dependent on the SecYEG translocon and YidC. Importantly, membrane insertion of proteins translated from membrane-bound mRNAs occurs independently of the SRP pathway, while the latter is strictly required for proteins translated from cytosolic mRNAs. In summary, our data indicate that mRNA targeting acts in parallel to the canonical SRP-dependent protein targeting and serves as an alternative strategy for safeguarding membrane protein insertion when the SRP pathway is compromised.
Identifiants
pubmed: 36842086
pii: S2211-1247(23)00151-1
doi: 10.1016/j.celrep.2023.112140
pmc: PMC10066597
pii:
doi:
Substances chimiques
Membrane Proteins
0
Signal Recognition Particle
0
Escherichia coli Proteins
0
Bacterial Proteins
0
RNA, Messenger
0
SEC Translocation Channels
0
YIDC protein, E coli
0
Membrane Transport Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
112140Informations de copyright
Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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