Application of CRISPR Cas systems in DNA recorders and writers.
CRISPR Cas systems
DNA recorders
DNA typewriter
DNA writers
PASTE
Prime editing
Journal
Bio Systems
ISSN: 1872-8324
Titre abrégé: Biosystems
Pays: Ireland
ID NLM: 0430773
Informations de publication
Date de publication:
Mar 2023
Mar 2023
Historique:
received:
04
12
2022
revised:
13
02
2023
accepted:
23
02
2023
pubmed:
27
2
2023
medline:
21
3
2023
entrez:
26
2
2023
Statut:
ppublish
Résumé
The necessity to record and store biological data is increasing in due course of time. However, it is quite difficult to understand biological mechanisms and keep a track of these events in some storage mediums. DNA (deoxyribonucleic acid) is the best candidate for the storage of cellular events in the biological system. It is energy efficient as well as stable at the same time. DNA-based writers and memory devices are continually evolving and finding new avenues in terms of their wide range of applications. Among all the DNA-based storage devices that employ enzymes like recombinases, nucleases, integrases, and polymerases, one of the most popular tools used for these devices is the emerging and versatile CRISPR Cas technology. CRISPR Cas is a prokaryotic immune system that keeps a memory of viral attacks and protects prokaryotes from potential future infections. The main aim of this short review is to study such molecular recorders and writers that employ CRISPR Cas technologies and obtain an in-depth overview of the mechanisms involved and the applications of these molecular devices.
Identifiants
pubmed: 36842456
pii: S0303-2647(23)00045-X
doi: 10.1016/j.biosystems.2023.104870
pii:
doi:
Substances chimiques
DNA
9007-49-2
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
104870Informations de copyright
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