The differential placental expression of ERp44 and pre-eclampsia; association with placental zinc, the ERAP1 and the renin-angiotensin-system.
Angiotensin
ERp44
Endoplasmic reticulum
Hypertension in pregnancy
Micronutrient
Placenta
Journal
Placenta
ISSN: 1532-3102
Titre abrégé: Placenta
Pays: Netherlands
ID NLM: 8006349
Informations de publication
Date de publication:
24 03 2023
24 03 2023
Historique:
received:
09
12
2022
revised:
06
02
2023
accepted:
21
02
2023
pubmed:
28
2
2023
medline:
15
3
2023
entrez:
27
2
2023
Statut:
ppublish
Résumé
Endoplasmic reticulum resident protein 44 (ERp44) is a zinc-metalloprotein, regulating Endoplasmic reticulum aminopeptidase 1 (ERAP1) and Angiotensin II (Ang II). We explored placental ERp44 expression and components of the renin-angiotensin-system (RAS) in pre-eclampsia (PE), correlating these to ERAP1 expression and placental zinc concentrations. Placental tissue, taken at time of delivery in normotensive women or women with PE (n = 12/group), were analysed for ERp44, AT1R, AT2R and AT4R by qPCR. Protein ERp44 expression was measured by immunohistochemistry and compared to previously measured ERAP1 expression. Placental zinc was measured by inductively-coupled-mass-spectrometry. ERp44 gene/protein expression were increased in PE (P < 0.05). AT1R expression was increased (P = 0.02) but AT4R decreased (P = 0.01) in PE, compared to normotensive controls. A positive association between ERp44 and AT2R expression was observed in all groups. ERp44 was negatively correlated with ERAP1 protein expression in all samples. Placental zinc concentrations were lower in women with PE (P = 0.001) and negatively associated with ERp44 gene expression. Increased placental ERp44 could further reduce ERAP1 release in PE, potentially preventing release of Ang IV and thus lowering levels of Ang IV which consequently diminishes the possibility of counterbalancing the activity of vasoconstrictive, Ang II. The lower placental zinc may contribute to dysfunction of the ERp44/ERAP1 complex, exacerbating the hypertension in PE.
Identifiants
pubmed: 36848863
pii: S0143-4004(23)00038-3
doi: 10.1016/j.placenta.2023.02.006
pmc: PMC10682376
pii:
doi:
Substances chimiques
Renin
EC 3.4.23.15
Zinc
J41CSQ7QDS
Angiotensin II
11128-99-7
ERP44 protein, human
0
Membrane Proteins
0
Molecular Chaperones
0
ERAP1 protein, human
EC 3.4.11.-
Aminopeptidases
EC 3.4.11.-
Minor Histocompatibility Antigens
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
9-14Subventions
Organisme : British Heart Foundation
ID : FS/15/32/31604
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/P027938/1
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 217123/Z/19/Z
Pays : United Kingdom
Organisme : Department of Health
Pays : United Kingdom
Informations de copyright
Crown Copyright © 2023. Published by Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest None.
Références
Matern Child Nutr. 2014 Jul;10(3):327-34
pubmed: 22784110
Free Radic Biol Med. 2015 Jun;83:323-30
pubmed: 25744412
Chem Rev. 2009 Oct;109(10):4682-707
pubmed: 19728700
Hypertens Pregnancy. 2001;20(1):IX-XIV
pubmed: 12044323
Placenta. 2010 May;31(5):448-55
pubmed: 20304486
Front Immunol. 2020 Mar 10;11:396
pubmed: 32210971
Arch Biochem Biophys. 1992 Feb 1;292(2):388-92
pubmed: 1731608
Nat Commun. 2019 Feb 5;10(1):603
pubmed: 30723194
Biochim Biophys Acta. 2007 Oct;1774(10):1247-53
pubmed: 17855175
Mol Cell. 2015 Jun 18;58(6):1015-27
pubmed: 25959394
Hypertension. 2011 Sep;58(3):497-504
pubmed: 21730298
BMJ. 2007 Nov 10;335(7627):974
pubmed: 17975258
J Physiol. 2016 Mar 1;594(5):1327-40
pubmed: 26574162
BMJ. 1995 Sep 2;311(7005):609-13
pubmed: 7663256
Placenta. 2008 Sep;29(9):798-801
pubmed: 18684503
Pharmacol Rep. 2006;58 Suppl:69-74
pubmed: 17332674
Hypertension. 2008 Nov;52(5):881-8
pubmed: 18852388
Nutrients. 2012 Jul;4(7):782-98
pubmed: 22852063
PLoS One. 2013 Aug 22;8(8):e72682
pubmed: 23991143
Acta Obstet Gynecol Scand. 1984;63(7):629-31
pubmed: 6097088
J Int Med Res. 2008 Nov-Dec;36(6):1335-41
pubmed: 19094444
Arch Med Res. 2002 Jan-Feb;33(1):40-7
pubmed: 11825630
Iran J Nurs Midwifery Res. 2010 Summer;15(3):120-4
pubmed: 21589774
Placenta. 2020 Apr;93:34-42
pubmed: 32250737
Am J Clin Nutr. 1987 Nov;46(5):763-71
pubmed: 3673925
J Biochem. 2000 Nov;128(5):755-62
pubmed: 11056387
Gynecol Obstet Invest. 2007;64(1):17-23
pubmed: 17199091
Int J Mol Sci. 2018 Mar 15;19(3):
pubmed: 29543776
EMBO J. 2002 Feb 15;21(4):835-44
pubmed: 11847130
Biol Trace Elem Res. 2003 Aug;94(2):105-12
pubmed: 12958401
Mol Cell. 2013 Oct 10;52(1):75-86
pubmed: 24076220
Am J Physiol Renal Physiol. 2016 Feb 15;310(4):F259-71
pubmed: 26608790
Lancet. 2010 Aug 21;376(9741):631-44
pubmed: 20598363
Front Med (Lausanne). 2020 Jun 12;7:270
pubmed: 32596247
Placenta. 2013 Feb;34(2):182-6
pubmed: 23246097
Biometals. 2001 Sep-Dec;14(3-4):271-313
pubmed: 11831461
Placenta. 2009 Mar;30 Suppl A:S43-8
pubmed: 19081132