Improving thermostability of Bacillus amyloliquefaciens alpha-amylase by multipoint mutations.

Bacillus amyloliquefaciens / genetics Bacillus Amino Acid Sequence alpha-Amylases / genetics Enzyme Stability Temperature Mutation
Alpha-amylase Bacillus amyloliquefaciens Thermostability Three-dimensional model

Journal

Biochemical and biophysical research communications
ISSN: 1090-2104
Titre abrégé: Biochem Biophys Res Commun
Pays: United States
ID NLM: 0372516

Informations de publication

Date de publication:
23 04 2023
Historique:
received: 14 02 2023
revised: 22 02 2023
accepted: 23 02 2023
pubmed: 2 3 2023
medline: 22 3 2023
entrez: 1 3 2023
Statut: ppublish

Résumé

The medium-temperature alpha-amylase of Bacillus amyloliquefaciens is widely used in the food and washing process. Enhancing the thermostability of alpha-amylases and investigating the mechanism of stability are important for enzyme industry development. The optimal temperature and pH of the wild-type BAA and mutant MuBAA (D28E/V118A/S187D/K370 N) were all 60 °C and 6.0, respectively. The mutant MuBAA showed better thermostability at 50 °C and 60 °C, with a specific activity of 206.61 U/mg, which was 99.1% greater than that of the wild-type. By analyzing predicted structures, the improving thermostability of the mutant MuBAA was mainly related to enhanced stabilization of a loop region in domain B via more calcium-binding sites and intramolecular interactions around Asp187. Furthermore, additional intramolecular interactions around sites 28 and 370 in domain A were also beneficial for improving thermostability. Additionally, the decrease of steric hindrance at the active cavity increased the specific activity of the mutant MuBAA. Improving the thermostability of BAA has theoretical reference values for the modification of alpha-amylases.

Identifiants

DOI: 10.1016/j.bbrc.2023.02.064 PMID: 36857902
pubmed: 36857902
pii: S0006-291X(23)00250-4
doi: 10.1016/j.bbrc.2023.02.064
pii:
doi:

Substances chimiques

alpha-Amylases EC 3.2.1.1

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

69-75

Informations de copyright

Copyright © 2023 Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Auteurs

Susu Yuan (S)

National Engineering Laboratory for High-efficient Enzyme Expression, Fuzhou, Fujian, China; The Key Laboratory of Marine Enzyme Engineering of Fujian Province, Fuzhou University, Fuzhou, Fujian, China; College of Biological Science and Engineering, Fuzhou University, Fuzhou, Fujian, China.

Renxiang Yan (R)

National Engineering Laboratory for High-efficient Enzyme Expression, Fuzhou, Fujian, China; The Key Laboratory of Marine Enzyme Engineering of Fujian Province, Fuzhou University, Fuzhou, Fujian, China; College of Biological Science and Engineering, Fuzhou University, Fuzhou, Fujian, China.

Biyu Lin (B)

National Engineering Laboratory for High-efficient Enzyme Expression, Fuzhou, Fujian, China; The Key Laboratory of Marine Enzyme Engineering of Fujian Province, Fuzhou University, Fuzhou, Fujian, China; College of Biological Science and Engineering, Fuzhou University, Fuzhou, Fujian, China.

Renkuan Li (R)

National Engineering Laboratory for High-efficient Enzyme Expression, Fuzhou, Fujian, China; The Key Laboratory of Marine Enzyme Engineering of Fujian Province, Fuzhou University, Fuzhou, Fujian, China; College of Biological Science and Engineering, Fuzhou University, Fuzhou, Fujian, China.

Xiuyun Ye (X)

National Engineering Laboratory for High-efficient Enzyme Expression, Fuzhou, Fujian, China; The Key Laboratory of Marine Enzyme Engineering of Fujian Province, Fuzhou University, Fuzhou, Fujian, China; College of Biological Science and Engineering, Fuzhou University, Fuzhou, Fujian, China. Electronic address: xiuyunye@fzu.edu.cn.

Articles similaires

Spatial multiplex analysis of lung cancer reveals that regulatory T cells attenuate KRAS-G12C inhibitor-induced immune responses.

Megan Cole, Panayiotis Anastasiou, Claudia Lee et al.
1.00
T-Lymphocytes, Regulatory Lung Neoplasms Proto-Oncogene Proteins p21(ras) Animals Humans

Pathogenic mitochondrial DNA mutations inhibit melanoma metastasis.

Spencer D Shelton, Sara House, Luiza Martins Nascentes Melo et al.
1.00
DNA, Mitochondrial Humans Melanoma Mutation Neoplasm Metastasis

Decoding the genomic terrain: functional insights into 14 chemosensory proteins in whitefly Bemisia tabaci Asia II-1.

M N Rudra Gouda, Sabtharishi Subramanian
1.00
Animals Hemiptera Insect Proteins Phylogeny Insecticides

Prevalence and implications of fragile X premutation screening in Thailand.

Areerat Hnoonual, Sunita Kaewfai, Chanin Limwongse et al.
1.00
Humans Fragile X Mental Retardation Protein Thailand Male Female

Classifications MeSH

questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets sporulés à Gram positif › Bacillaceae › Bacillus › Bacillus amyloliquefaciens › Improving thermostability of Bacillus...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets sporulés à Gram positif › Bacillaceae › Bacillus › Improving thermostability of Bacillus...
questionsmedicales.fr › Sciences de l'information › Sources d'information › Services d'information › Documentation › Données de séquences moléculaires › Séquence d'acides aminés › Improving thermostability of Bacillus...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Glycosidases › Amylases › alpha-Amylases › Improving thermostability of Bacillus...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Stabilité protéique › Stabilité enzymatique › Improving thermostability of Bacillus...
questionsmedicales.fr › Environnement et santé publique › Environnement › Concepts météorologiques › Atmosphère › Temps (météorologie) › Température › Improving thermostability of Bacillus...
questionsmedicales.fr › Phénomènes génétiques › Variation génétique › Mutation › Improving thermostability of Bacillus...