Identification and degradation of structural extracellular polymeric substances in waste activated sludge via a polygalacturonate-degrading consortium.

Sewage / chemistry Waste Disposal, Fluid / methods Extracellular Polymeric Substance Matrix Methane Anaerobiosis
Anaerobic digestion Clostridium Pectate lyases Polygalacturonate-degrading consortium Structural extracellular polymeric substances Waste activated sludge

Journal

Water research
ISSN: 1879-2448
Titre abrégé: Water Res
Pays: England
ID NLM: 0105072

Informations de publication

Date de publication:
15 Apr 2023
Historique:
received: 05 12 2022
revised: 22 02 2023
accepted: 22 02 2023
pubmed: 4 3 2023
medline: 16 3 2023
entrez: 3 3 2023
Statut: ppublish

Résumé

By maintaining the cell integrity of waste activated sludge (WAS), structural extracellular polymeric substances (St-EPS) resist WAS anaerobic fermentation. This study investigates the occurrence of polygalacturonate in WAS St-EPS by combining chemical and metagenomic analyses that identify ∼22% of the bacteria, including Ferruginibacter and Zoogloea, that are associated with polygalacturonate production using the key enzyme EC 5.1.3.6. A highly active polygalacturonate-degrading consortium (GDC) was enriched and the potential of this GDC for degrading St-EPS and promoting methane production from WAS was investigated. The percentage of St-EPS degradation increased from 47.6% to 85.2% after inoculation with the GDC. Methane production was also increased by up to 2.3 times over a control group, with WAS destruction increasing from 11.5% to 28.4%. Zeta potential and rheological behavior confirmed the positive effect which GDC has on WAS fermentation. The major genus in the GDC was identified as Clostridium (17.1%). Extracellular pectate lyases (EC 4.2.2.2 and 4.2.2.9), excluding polygalacturonase (EC 3.2.1.15), were observed in the metagenome of the GDC and most likely play a core role in St-EPS hydrolysis. Dosing with GDC provides a good biological method for St-EPS degradation and thereby enhances the conversion of WAS to methane.

Identifiants

DOI: 10.1016/j.watres.2023.119800 PMID: 36868117
pubmed: 36868117
pii: S0043-1354(23)00235-X
doi: 10.1016/j.watres.2023.119800
pii:
doi:

Substances chimiques

Sewage 0
polygalacturonic acid VV3XD4CL04
Methane OP0UW79H66

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

119800

Informations de copyright

Copyright © 2023 Elsevier Ltd. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Auteurs

Zhi-Yi Hu (ZY)

Engineering Research Center of Soil Remediation of Fujian Province University, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

Yi-Peng Lin (YP)

Engineering Research Center of Soil Remediation of Fujian Province University, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

Qing-Ting Wang (QT)

Engineering Research Center of Soil Remediation of Fujian Province University, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

Yi-Xin Zhang (YX)

Engineering Research Center of Soil Remediation of Fujian Province University, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

Jie Tang (J)

Engineering Research Center of Soil Remediation of Fujian Province University, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

Si-Di Hong (SD)

Engineering Research Center of Soil Remediation of Fujian Province University, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

Kun Dai (K)

Engineering Research Center of Soil Remediation of Fujian Province University, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

Shuai Wang (S)

Engineering Research Center of Soil Remediation of Fujian Province University, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

Yong-Ze Lu (YZ)

School of Energy and Environment, Southeast University, Nanjing 210096, China.

Mark C M van Loosdrecht (MCM)

Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, the Netherlands.

Jianrong Wu (J)

Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.

Raymond Jianxiong Zeng (RJ)

Engineering Research Center of Soil Remediation of Fujian Province University, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China. Electronic address: rzeng@ustc.edu.cn.

Fang Zhang (F)

Engineering Research Center of Soil Remediation of Fujian Province University, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China. Electronic address: zhfang@mail.ustc.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Mélanges complexes › Eaux d'égout › Identification and degradation of structural...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Amélioration du niveau sanitaire › Génie sanitaire › Gestion des déchets › Élimination des déchets › Élimination des déchets liquides › Identification and degradation of structural...
questionsmedicales.fr › Phénomènes microbiologiques › Biofilms › Matrice de substances polymériques extracellulaires › Identification and degradation of structural...
questionsmedicales.fr › Composés chimiques organiques › Hydrocarbures › Hydrocarbures acycliques › Alcanes › Méthane › Identification and degradation of structural...
questionsmedicales.fr › Métabolisme › Anaérobiose › Identification and degradation of structural...