Enhancement in the catalytic efficiency of D-amino acid oxidase from Glutamicibacter protophormiae by multiple amino acid substitutions.

Amino Acid Substitution Amino Acids Catalytic Domain Kinetics Mutagenesis, Site-Directed Substrate Specificity D-Amino-Acid Oxidase
Active-site lid Catalytic efficiency D-amino acid oxidase Glutamicibacter protophormia Triple-point mutant

Journal

Enzyme and microbial technology
ISSN: 1879-0909
Titre abrégé: Enzyme Microb Technol
Pays: United States
ID NLM: 8003761

Informations de publication

Date de publication:
May 2023
Historique:
received: 21 10 2022
revised: 16 01 2023
accepted: 27 02 2023
medline: 11 4 2023
pubmed: 9 3 2023
entrez: 8 3 2023
Statut: ppublish

Résumé

D-Amino acid oxidase (DAAO) is an imperative oxidoreductase that oxidizes D-amino acids to corresponding keto acids, producing ammonia and hydrogen peroxide. Previously, based on the sequence alignment of DAAO from Glutamicibacter protophormiae (GpDAAO-1) and (GpDAAO-2), 4 residues (E115, N119, T256, T286) at the surface regions of GpDAAO-2, were subjected to site-directed mutagenesis and achieved 4 single-point mutants with enhanced catalytic efficiency (k

Identifiants

DOI: 10.1016/j.enzmictec.2023.110224 PMID: 36889103
pubmed: 36889103
pii: S0141-0229(23)00032-7
doi: 10.1016/j.enzmictec.2023.110224
pii:
doi:

Substances chimiques

Amino Acids 0
D-Amino-Acid Oxidase EC 1.4.3.3

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

110224

Informations de copyright

Copyright © 2023 Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Conflict of interest The authors declare no conflict of interest regarding the data presented in the manuscript.

Auteurs

Shujing Xu (S)

College of Life Science, Hebei Normal University, Shijiazhuang 050024, China.

Mengqiu Chu (M)

College of Life Science, Hebei Normal University, Shijiazhuang 050024, China; Hebei Collaborative Innovation Center for Eco-Environment, Shijiazhuang 050024, China.

Fa Zhang (F)

College of Life Science, Hebei Normal University, Shijiazhuang 050024, China.

Jiawei Zhao (J)

College of Life Science, Hebei Normal University, Shijiazhuang 050024, China.

Jiaqi Zhang (J)

College of Life Science, Hebei Normal University, Shijiazhuang 050024, China.

Yuting Cao (Y)

College of Life Science, Hebei Normal University, Shijiazhuang 050024, China.

Guangzheng He (G)

College of Life Science, Hebei Normal University, Shijiazhuang 050024, China.

Muhammad Israr (M)

Department of Biology, The University of Haripur, Haripur 22620, Pakistan. Electronic address: m.israr@uoh.edu.pk.

Baohua Zhao (B)

College of Life Science, Hebei Normal University, Shijiazhuang 050024, China.

Jiansong Ju (J)

College of Life Science, Hebei Normal University, Shijiazhuang 050024, China; Hebei Collaborative Innovation Center for Eco-Environment, Shijiazhuang 050024, China. Electronic address: jujiansong@126.com.

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Classifications MeSH

questionsmedicales.fr › Phénomènes génétiques › Mutagenèse › Substitution d'acide aminé › Enhancement in the catalytic efficiency of...
questionsmedicales.fr › Acides aminés, peptides et protéines › Acides aminés › Enhancement in the catalytic efficiency of...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Éléments structuraux des protéines › Domaine catalytique › Enhancement in the catalytic efficiency of...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Cinétique › Enhancement in the catalytic efficiency of...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Génie génétique › Ingénierie des protéines › Mutagenèse dirigée › Enhancement in the catalytic efficiency of...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Spécificité du substrat › Enhancement in the catalytic efficiency of...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Oxidoreductases › Oxidoreductases acting on CH-NH2 group donors › Amino-acid oxidoreductases › D-amino-acid oxidase › Enhancement in the catalytic efficiency of...