Deuterium isotope probing (DIP) on Listeria innocua: Optimisation of labelling and impact on viability state.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2023
2023
Historique:
received:
16
03
2022
accepted:
10
01
2023
entrez:
9
3
2023
pubmed:
10
3
2023
medline:
14
3
2023
Statut:
epublish
Résumé
An innovative approach, Raman microspectroscopy coupled with deuterium isotope probing (Raman-DIP), can be used to evaluate the metabolism of deuterated carbon source in bacteria and also to presume different anabolic pathways. This method requires the treatment of cells with heavy water that could affect the bacterial viability state at higher concentration. In this study, we evaluated the effect of heavy water incorporation on the viability state of Listeria innocua cells. We exposed the L. innocua suspensions to different heavy water concentrations (0%, 25%, 50% and 75%) from 30 minutes to 72 h of incubation times at 37°C. The total, viable and viable culturable populations were quantified by qPCR, PMA-qPCR and plate count agar respectively. We analyzed heavy water incorporation by Raman-DIP. The exposure of L. innocua cells to different concentrations of heavy water did not alter their cell viability to 24 h incubation time. In addition, the maximum intensity for C-D band, specific for the incorporation of heavy water, was reached after 2 h of exposure in a media containing 75% v/v D2O but an early detection of the labelling was possible at t = 1 h 30 min. In conclusion, the use of D2O as a metabolic marker was validated and can be developed for the detection of L. innocua cell viability state.
Identifiants
pubmed: 36893178
doi: 10.1371/journal.pone.0280885
pii: PONE-D-22-07800
pmc: PMC9997870
doi:
Substances chimiques
Deuterium
AR09D82C7G
Deuterium Oxide
J65BV539M3
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0280885Informations de copyright
Copyright: © 2023 Trigueros et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Appl Microbiol Biotechnol. 2016 Jun;100(12):5273-87
pubmed: 27129530
Lett Appl Microbiol. 2000 Jul;31(1):77-81
pubmed: 10886620
ISRN Microbiol. 2013 Sep 26;2013:703813
pubmed: 24191231
Foods. 2022 May 22;11(10):
pubmed: 35627076
FEMS Microbiol Rev. 2010 Jul;34(4):415-25
pubmed: 20059548
Proc Natl Acad Sci U S A. 2015 Jan 13;112(2):E194-203
pubmed: 25550518
Anal Chem. 2017 Dec 19;89(24):13305-13312
pubmed: 29135226
Int J Food Microbiol. 2004 Feb 1;90(3):341-7
pubmed: 14751689
Bull Tokyo Dent Coll. 2010;51(4):175-83
pubmed: 21139370
EFSA J. 2021 Dec 13;19(12):e06971
pubmed: 36329690
Food Sci Technol Int. 2020 Apr;26(3):205-212
pubmed: 31583896
FEMS Microbiol Rev. 2005 Nov;29(5):851-75
pubmed: 16219509
J Microbiol Methods. 2011 May;85(2):164-9
pubmed: 21324348
J Biomed Opt. 2014;19(11):111610
pubmed: 25028774
Vet Res. 2002 Jul-Aug;33(4):359-70
pubmed: 12199363
Environ Int. 2019 Sep;130:104883
pubmed: 31229870
J Appl Microbiol. 2001 Jun;90(6):1000-5
pubmed: 11412331
Food Microbiol. 2020 Dec;92:103548
pubmed: 32950145
Folia Microbiol (Praha). 2019 Sep;64(5):673-681
pubmed: 31352668
J Clin Microbiol. 2003 Nov;41(11):5308-9
pubmed: 14605191