Computational modeling of cambium activity provides a regulatory framework for simulating radial plant growth.

A. thaliana computational biology organ growth plant biology stem cell regulation wood formation

Journal

eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614

Informations de publication

Date de publication:
10 03 2023
Historique:
received: 17 01 2021
accepted: 06 03 2023
medline: 5 4 2023
pubmed: 11 3 2023
entrez: 10 3 2023
Statut: epublish

Résumé

Precise organization of growing structures is a fundamental process in developmental biology. In plants, radial growth is mediated by the cambium, a stem cell niche continuously producing wood (xylem) and bast (phloem) in a strictly bidirectional manner. While this process contributes large parts to terrestrial biomass, cambium dynamics eludes direct experimental access due to obstacles in live-cell imaging. Here, we present a cell-based computational model visualizing cambium activity and integrating the function of central cambium regulators. Performing iterative comparisons of plant and model anatomies, we conclude that the receptor-like kinase PXY and its ligand CLE41 are part of a minimal framework sufficient for instructing tissue organization. By integrating tissue-specific cell wall stiffness values, we moreover probe the influence of physical constraints on tissue geometry. Our model highlights the role of intercellular communication within the cambium and shows that a limited number of factors are sufficient to create radial growth by bidirectional tissue production.

Identifiants

pubmed: 36897801
doi: 10.7554/eLife.66627
pii: 66627
pmc: PMC10069871
doi:
pii:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

© 2023, Lebovka et al.

Déclaration de conflit d'intérêts

IL, BH, XL, AZ, TS, NG, RM, RG, TG No competing interests declared

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Auteurs

Ivan Lebovka (I)

Centre for Organismal Studies, Heidelberg University, Heidelberg, Germany.

Bruno Hay Mele (B)

Department of Agricultural Sciences, Università degli Studi di Napoli Federico II, Napoli, Italy.

Xiaomin Liu (X)

Centre for Organismal Studies, Heidelberg University, Heidelberg, Germany.

Alexandra Zakieva (A)

Centre for Organismal Studies, Heidelberg University, Heidelberg, Germany.

Theresa Schlamp (T)

Centre for Organismal Studies, Heidelberg University, Heidelberg, Germany.

Nial Rau Gursanscky (NR)

Gregor Mendel Institute, Vienna Biocenter, Vienna, Austria.

Roeland M H Merks (RMH)

Mathematical Institute, Leiden University, Leiden, Netherlands.
Institute of Biology, Leiden University, Leiden, Netherlands.

Ruth Großeholz (R)

Centre for Organismal Studies, Heidelberg University, Heidelberg, Germany.
BioQuant, Heidelberg University, Heidelberg, Germany.

Thomas Greb (T)

Centre for Organismal Studies, Heidelberg University, Heidelberg, Germany.

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Classifications MeSH