Extensive Evaluation of a Method for Quantitative Measurement of Aflatoxins B1 and M1 in Animal Urine Using High-Performance Liquid Chromatography with Fluorescence Detection.
Journal
Journal of AOAC International
ISSN: 1944-7922
Titre abrégé: J AOAC Int
Pays: England
ID NLM: 9215446
Informations de publication
Date de publication:
03 May 2023
03 May 2023
Historique:
received:
23
11
2022
revised:
17
01
2023
accepted:
31
01
2023
medline:
5
5
2023
pubmed:
14
3
2023
entrez:
13
3
2023
Statut:
ppublish
Résumé
Aflatoxins (AFs) are common feed contaminants and are one of the common causes of toxin-related pet food poisoning and recalls. Currently, there are no validated methods for the detection and quantitation of AFs in biological matrices to diagnose AF exposure in live animals. Following a successful intra-laboratory method development to quantify AFB1 and AFM1 in animal urine by HPLC with fluorescence detection (HPLC-FLD), the present study was conducted to extensively evaluate the method performance in an unbiased manner using blinded samples. The evaluation included two stages. First, the performance was verified in the method-originating laboratory in a single-laboratory blinded method test (BMT-S) trial followed by a multi-laboratory blinded method test (BMT-M) trial. In both trials, accuracy, repeatability, and reproducibility were satisfactory confirming the relatively good ruggedness and robustness of the method and ensuring that it will perform as expected if used by other laboratories in the future. We extensively evaluated the performance of a quantitative method to detect AFB1 and AFM1 in animal urine by HPLC-FLD by two different laboratories in two separate BMT-S and BMT-M trials. Both BMT results demonstrated the satisfactory accuracy and precision of the method. It is now available to be adopted by other diagnostic laboratories for purposes of diagnosing AF intoxication in animals. A simple urine-based diagnostic test method using HPLC-FLD that originated in a single laboratory now has passed a multi-laboratory evaluation and is now available to be shared with other diagnostic laboratories for purposes of diagnosing AF intoxication in animals so better treatment can be rendered.
Sections du résumé
BACKGROUND
BACKGROUND
Aflatoxins (AFs) are common feed contaminants and are one of the common causes of toxin-related pet food poisoning and recalls.
OBJECTIVE
OBJECTIVE
Currently, there are no validated methods for the detection and quantitation of AFs in biological matrices to diagnose AF exposure in live animals. Following a successful intra-laboratory method development to quantify AFB1 and AFM1 in animal urine by HPLC with fluorescence detection (HPLC-FLD), the present study was conducted to extensively evaluate the method performance in an unbiased manner using blinded samples.
METHODS
METHODS
The evaluation included two stages. First, the performance was verified in the method-originating laboratory in a single-laboratory blinded method test (BMT-S) trial followed by a multi-laboratory blinded method test (BMT-M) trial.
RESULTS
RESULTS
In both trials, accuracy, repeatability, and reproducibility were satisfactory confirming the relatively good ruggedness and robustness of the method and ensuring that it will perform as expected if used by other laboratories in the future.
CONCLUSIONS
CONCLUSIONS
We extensively evaluated the performance of a quantitative method to detect AFB1 and AFM1 in animal urine by HPLC-FLD by two different laboratories in two separate BMT-S and BMT-M trials. Both BMT results demonstrated the satisfactory accuracy and precision of the method. It is now available to be adopted by other diagnostic laboratories for purposes of diagnosing AF intoxication in animals.
HIGHLIGHTS
CONCLUSIONS
A simple urine-based diagnostic test method using HPLC-FLD that originated in a single laboratory now has passed a multi-laboratory evaluation and is now available to be shared with other diagnostic laboratories for purposes of diagnosing AF intoxication in animals so better treatment can be rendered.
Identifiants
pubmed: 36912688
pii: 7076595
doi: 10.1093/jaoacint/qsad034
pmc: PMC10156413
doi:
Substances chimiques
Aflatoxin B1
9N2N2Y55MH
Aflatoxins
0
Types de publication
Clinical Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
645-651Subventions
Organisme : FDA HHS
ID : U18 FD005006
Pays : United States
Informations de copyright
© The Author(s) 2023. Published by Oxford University Press on behalf of AOAC INTERNATIONAL.
Références
Annu Rev Pharmacol Toxicol. 1994;34:135-72
pubmed: 8042848
Cancer Res. 1969 Dec;29(12):2206-11
pubmed: 4318833
Morphol Embryol (Bucur). 1980 Oct-Dec;26(4):309-14
pubmed: 6450879
Front Microbiol. 2019 Oct 04;10:2266
pubmed: 31636616
Int J Cancer. 2005 Jul 20;115(6):879-84
pubmed: 15723309
Toxins (Basel). 2021 Apr 17;13(4):
pubmed: 33920591
Mycopathologia. 1993 Dec;124(3):189-94
pubmed: 8022466
J AOAC Int. 2021 Jun 12;104(3):546-554
pubmed: 33394021
J AOAC Int. 2019 Sep 1;102(5):1530-1534
pubmed: 30736868
Cancer Res. 1971 Dec;31(12):1936-42
pubmed: 4330435
J AOAC Int. 2006 Jul-Aug;89(4):1095-109
pubmed: 16915851
Environ Health Perspect. 2010 Jun;118(6):818-24
pubmed: 20172840
Int J Environ Res Public Health. 2019 Sep 27;16(19):
pubmed: 31569703
J Chromatogr A. 2000 Jun 16;882(1-2):1-10
pubmed: 10895926
Environ Health Perspect. 1996 Mar;104 Suppl 1:5-21
pubmed: 8722107
J Anal Toxicol. 2017 Oct 1;41(8):698-707
pubmed: 28985321
J AOAC Int. 2000 Mar-Apr;83(2):399-406
pubmed: 10772178