Spt6 directly interacts with Cdc73 and is required for Paf1 complex occupancy at active genes in Saccharomyces cerevisiae.
Journal
Nucleic acids research
ISSN: 1362-4962
Titre abrégé: Nucleic Acids Res
Pays: England
ID NLM: 0411011
Informations de publication
Date de publication:
09 06 2023
09 06 2023
Historique:
accepted:
28
02
2023
revised:
21
02
2023
received:
29
04
2022
medline:
12
6
2023
pubmed:
18
3
2023
entrez:
17
3
2023
Statut:
ppublish
Résumé
The Paf1 complex (Paf1C) is a conserved transcription elongation factor that regulates transcription elongation efficiency, facilitates co-transcriptional histone modifications, and impacts molecular processes linked to RNA synthesis, such as polyA site selection. Coupling of the activities of Paf1C to transcription elongation requires its association with RNA polymerase II (Pol II). Mutational studies in yeast identified Paf1C subunits Cdc73 and Rtf1 as important mediators of Paf1C association with Pol II on active genes. While the interaction between Rtf1 and the general elongation factor Spt5 is relatively well-understood, the interactions involving Cdc73 have not been fully elucidated. Using a site-specific protein cross-linking strategy in yeast cells, we identified direct interactions between Cdc73 and two components of the Pol II elongation complex, the elongation factor Spt6 and the largest subunit of Pol II. Both of these interactions require the tandem SH2 domain of Spt6. We also show that Cdc73 and Spt6 can interact in vitro and that rapid depletion of Spt6 dissociates Paf1 from chromatin, altering patterns of Paf1C-dependent histone modifications genome-wide. These results reveal interactions between Cdc73 and the Pol II elongation complex and identify Spt6 as a key factor contributing to the occupancy of Paf1C at active genes in Saccharomyces cerevisiae.
Identifiants
pubmed: 36928138
pii: 7079632
doi: 10.1093/nar/gkad180
pmc: PMC10250246
doi:
Substances chimiques
Nuclear Proteins
0
Peptide Elongation Factors
0
RNA Polymerase II
EC 2.7.7.-
Saccharomyces cerevisiae Proteins
0
Transcription Factors
0
Transcriptional Elongation Factors
0
SPT6 protein, S cerevisiae
0
RTF1 protein, S cerevisiae
0
PAF1 protein, S cerevisiae
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
4814-4830Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM052593
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM141964
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM137905
Pays : United States
Organisme : NCI NIH HHS
ID : R21 CA261737
Pays : United States
Organisme : NIGMS NIH HHS
ID : F31 GM129917
Pays : United States
Informations de copyright
© The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.
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