Development of probiotic E. coli Nissle 1917 for β-alanine production by using protein and metabolic engineering.
Aspartate-α-decarboxylase
Escherichia coli Nissle 1917
Fed-batch fermentation
Genetic engineering
β-Alanine
Journal
Applied microbiology and biotechnology
ISSN: 1432-0614
Titre abrégé: Appl Microbiol Biotechnol
Pays: Germany
ID NLM: 8406612
Informations de publication
Date de publication:
Apr 2023
Apr 2023
Historique:
received:
09
09
2022
accepted:
06
03
2023
revised:
18
01
2023
pubmed:
18
3
2023
medline:
25
3
2023
entrez:
17
3
2023
Statut:
ppublish
Résumé
β-alanine has been used in food and pharmaceutical industries. Although Escherichia coli Nissle 1917 (EcN) is generally considered safe and engineered as living therapeutics, engineering EcN for producing industrial metabolites has rarely been explored. Here, by protein and metabolic engineering, EcN was engineered for producing β-alanine from glucose. First, an aspartate-α-decarboxylase variant ADC
Identifiants
pubmed: 36929190
doi: 10.1007/s00253-023-12477-5
pii: 10.1007/s00253-023-12477-5
doi:
Substances chimiques
beta-Alanine
11P2JDE17B
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2277-2288Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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