A MAD7-based genome editing system for Escherichia coli.
Journal
Microbial biotechnology
ISSN: 1751-7915
Titre abrégé: Microb Biotechnol
Pays: United States
ID NLM: 101316335
Informations de publication
Date de publication:
05 2023
05 2023
Historique:
revised:
29
01
2023
received:
28
09
2022
accepted:
31
01
2023
medline:
27
4
2023
pubmed:
18
3
2023
entrez:
17
3
2023
Statut:
ppublish
Résumé
A broad variety of biomolecules is industrially produced in bacteria and yeasts. These microbial expression hosts can be optimized through genetic engineering using CRISPR tools. Here, we designed and characterized such a modular genome editing system based on the Cas12a-like RNA-guided nuclease MAD7 in Escherichia coli. This system enables the efficient generation of single nucleotide polymorphisms (SNPs) or gene deletions and can directly be used with donor DNA from benchtop DNA assembly to increase throughput. We combined multiple edits to engineer an E. coli strain with reduced overflow metabolism and increased plasmid yield, highlighting the versatility and industrial applicability of this approach.
Identifiants
pubmed: 36929689
doi: 10.1111/1751-7915.14234
pmc: PMC10128132
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1000-1010Informations de copyright
© 2023 Sanofi Aventis Deutschland GmbH. Microbial Biotechnology published by Applied Microbiology International and John Wiley & Sons Ltd.
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