A Tri-Enzyme Cascade for Efficient Production of L-2-Aminobutyrate from L-Threonine.
L-2-aminobutyrate
leucine dehydrogenase
multi-enzyme cascade
protein engineering
Journal
Chembiochem : a European journal of chemical biology
ISSN: 1439-7633
Titre abrégé: Chembiochem
Pays: Germany
ID NLM: 100937360
Informations de publication
Date de publication:
01 08 2023
01 08 2023
Historique:
revised:
20
03
2023
received:
23
02
2023
medline:
2
8
2023
pubmed:
23
3
2023
entrez:
22
3
2023
Statut:
ppublish
Résumé
L-2-aminobutyrate (L-ABA) is an important chiral drug intermediate with a key role in modern medicinal chemistry. Here, we describe the development of an efficient method for the asymmetric synthesis of L-ABA in a tri-enzymatic cascade in Escherichia coli BL21 (DE3) using a cost-effective L-Thr. Low activity of leucine dehydrogenase from Bacillus thuringiensis (BtLDH) and unbalanced expression of enzymes in the cascade were major challenges. Mechanism-based protein engineering generated the optimal triple variant BtLDH
Identifiants
pubmed: 36946691
doi: 10.1002/cbic.202300148
doi:
Substances chimiques
Threonine
2ZD004190S
Aminobutyrates
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e202300148Informations de copyright
© 2023 Wiley-VCH GmbH.
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