Targeted Modifications of the Yeast Genome to Study Peroxisomes.
Conditional gene/protein expression
Gene deletion
PCR-based tagging
S. cerevisiae
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2023
2023
Historique:
entrez:
23
3
2023
pubmed:
24
3
2023
medline:
28
3
2023
Statut:
ppublish
Résumé
PCR-based gene targeting enables rapid alteration of the Saccharomyces cerevisiae genome. Here we describe how this method can be applied for directed gene deletions, epitope and fluorescence protein tagging, and conditional gene expression, with a specific focus on peroxisomal proteins.
Identifiants
pubmed: 36952189
doi: 10.1007/978-1-0716-3048-8_16
doi:
Substances chimiques
Saccharomyces cerevisiae Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
217-232Informations de copyright
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
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