Efficient isolation of rare B cells using next-generation antigen barcoding.
AIRR
BCR
antibody
humoral immunology
immunology
multi-omics
single cell
Journal
Frontiers in cellular and infection microbiology
ISSN: 2235-2988
Titre abrégé: Front Cell Infect Microbiol
Pays: Switzerland
ID NLM: 101585359
Informations de publication
Date de publication:
2022
2022
Historique:
received:
06
06
2022
accepted:
28
12
2022
medline:
28
3
2023
entrez:
27
3
2023
pubmed:
28
3
2023
Statut:
epublish
Résumé
The ability to efficiently isolate antigen-specific B cells in high throughput will greatly accelerate the discovery of therapeutic monoclonal antibodies (mAbs) and catalyze rational vaccine development. Traditional mAb discovery is a costly and labor-intensive process, although recent advances in single-cell genomics using emulsion microfluidics allow simultaneous processing of thousands of individual cells. Here we present a streamlined method for isolation and analysis of large numbers of antigen-specific B cells, including next generation antigen barcoding and an integrated computational framework for B cell multi-omics. We demonstrate the power of this approach by recovering thousands of antigen-specific mAbs, including the efficient isolation of extremely rare precursors of VRC01-class and IOMA-class broadly neutralizing HIV mAbs.
Identifiants
pubmed: 36968243
doi: 10.3389/fcimb.2022.962945
pmc: PMC10036767
doi:
Substances chimiques
Antibodies, Neutralizing
0
HIV Antibodies
0
Antigens
0
Antibodies, Monoclonal
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
962945Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM133682
Pays : United States
Organisme : NIAID NIH HHS
ID : U19 AI135995
Pays : United States
Organisme : NIAID NIH HHS
ID : UM1 AI144462
Pays : United States
Organisme : NIAID NIH HHS
ID : T32 AI007244
Pays : United States
Informations de copyright
Copyright © 2023 Hurtado, Flynn, Lee, Salcedo, Cottrell, Skog, Burton, Nemazee, Schief, Landais, Sok and Briney.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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