Surfaceome Profiling Identifies Basigin-Chaperoned Protein Clients.
cell surface
chaperone
proteomics
surfaceome
trafficking
Journal
Chembiochem : a European journal of chemical biology
ISSN: 1439-7633
Titre abrégé: Chembiochem
Pays: Germany
ID NLM: 100937360
Informations de publication
Date de publication:
17 07 2023
17 07 2023
Historique:
revised:
21
03
2023
received:
30
01
2023
pmc-release:
17
07
2024
medline:
18
7
2023
pubmed:
28
3
2023
entrez:
27
3
2023
Statut:
ppublish
Résumé
The surface proteome or "surfaceome" is a critical mediator of cellular biology, facilitating cell-to-cell interactions and communication with extracellular biomolecules. Constituents of the surfaceome can serve as biomarkers for changing cell states and as targets for pharmacological intervention. While some pathways of cell surface trafficking are well characterized to allow prediction of surface localization, some non-canonical trafficking mechanisms do not. Basigin (Bsg), a cell surface glycoprotein, has been shown to chaperone protein clients to the cell surface. However, understanding which proteins are served by Bsg is not always straightforward. To accelerate such identification, we applied a surfaceome proximity labeling method that is integrated with quantitative mass spectrometry-based proteomics to discern changes in the surfaceome of hepatic stellate cells that occur in response to the genetic loss of Bsg. Using this strategy, we observed that the loss of Bsg leads to corresponding reductions in the cell surface expression of monocarboxylate transporters MCT1 and MCT4. We also found that these relationships were unique to Bsg and not found in neuroplastin (Nptn), a related family member. These results establish the utility of the surfaceome proximity labeling method to determine clients of cell surface chaperone proteins.
Identifiants
pubmed: 36973167
doi: 10.1002/cbic.202300073
pmc: PMC10424708
mid: NIHMS1921343
doi:
Substances chimiques
Basigin
136894-56-9
Membrane Glycoproteins
0
Membrane Proteins
0
Molecular Chaperones
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e202300073Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM142462
Pays : United States
Organisme : NIDDK NIH HHS
ID : R56 DK126895
Pays : United States
Organisme : NIDDK NIH HHS
ID : R56126895
Pays : United States
Informations de copyright
© 2023 The Authors. ChemBioChem published by Wiley-VCH GmbH.
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