Mechanistic insights into Schizosaccharomyces pombe GT-A family protein Pvg3 in the biosynthesis of pyruvylated β1,3-galactose of N-linked oligosaccharides.

Schizosaccharomyces / metabolism Galactose / metabolism Oligosaccharides / metabolism Polysaccharides / metabolism Galactosyltransferases / genetics
Fission yeast GT-A family Galactosyltransferase N-glycan Pyruvylated galactose

Journal

Journal of bioscience and bioengineering
ISSN: 1347-4421
Titre abrégé: J Biosci Bioeng
Pays: Japan
ID NLM: 100888800

Informations de publication

Date de publication:
Jun 2023
Historique:
received: 09 01 2023
revised: 24 02 2023
accepted: 04 03 2023
medline: 29 5 2023
pubmed: 1 4 2023
entrez: 31 3 2023
Statut: ppublish

Résumé

N-linked oligosaccharides in the fission yeast Schizosaccharomyces pombe contain large amounts of d-galactose (Gal), which mainly comprises α1,2- and α1,3-linked Gal except for pyruvylated β1,3-linked Gal (PvGalβ) at the non-reducing end. The PvGalβ unit of N-glycans is important for regulating nonsexual flocculation and invasive growth, but the mechanistic basis for β-galactosylation in fission yeast is poorly understood. To gain insight into this mechanism, we have characterized three genes previously identified to be involved in PvGalβ biosynthesis (pvg2, pvg3, and pvg5), with a focus on pvg3, which is predicted to contain a domain conserved in galactosyltransferase family 31 (GT31) proteins. Fluorescent microscopy revealed that Pvg3 is stably localized at the Golgi membrane, regardless of the presence of pvg2

Identifiants

DOI: 10.1016/j.jbiosc.2023.03.002 PMID: 37002018
pubmed: 37002018
pii: S1389-1723(23)00074-9
doi: 10.1016/j.jbiosc.2023.03.002
pii:
doi:

Substances chimiques

Galactose X2RN3Q8DNE
Oligosaccharides 0
Polysaccharides 0
Galactosyltransferases EC 2.4.1.-

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

423-432

Informations de copyright

Copyright © 2023 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

Auteurs

Takamasa Fukunaga (T)

Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka 819-0395, Japan.

Masahiro Watanabe (M)

Research Institute for Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-0046, Japan.

Yusuke Nakamichi (Y)

Research Institute for Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-0046, Japan.

Tomotake Morita (T)

Research Institute for Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-0046, Japan.

Yujiro Higuchi (Y)

Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka 819-0395, Japan.

Hiromi Maekawa (H)

Research Institute for Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-0046, Japan.

Kaoru Takegawa (K)

Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka 819-0395, Japan. Electronic address: takegawa@agr.kyushu-u.ac.jp.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Champignons › Levures › Schizosaccharomyces › Mechanistic insights into Schizosaccharomyces...
questionsmedicales.fr › Glucides › Sucres › Oses › Hexose › Galactose › Mechanistic insights into Schizosaccharomyces...
questionsmedicales.fr › Glucides › Polyosides › Oligosaccharides › Mechanistic insights into Schizosaccharomyces...
questionsmedicales.fr › Glucides › Polyosides › Mechanistic insights into Schizosaccharomyces...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Transferases › Glycosyltransferase › Hexosyltransferases › Galactosyltransferases › Mechanistic insights into Schizosaccharomyces...