Activation of actin-depolymerizing factor by CDPK16-mediated phosphorylation promotes actin turnover in Arabidopsis pollen tubes.
Journal
PLoS biology
ISSN: 1545-7885
Titre abrégé: PLoS Biol
Pays: United States
ID NLM: 101183755
Informations de publication
Date de publication:
04 2023
04 2023
Historique:
received:
15
09
2021
accepted:
11
03
2023
revised:
13
04
2023
medline:
17
4
2023
pubmed:
4
4
2023
entrez:
3
4
2023
Statut:
epublish
Résumé
As the stimulus-responsive mediator of actin dynamics, actin-depolymerizing factor (ADF)/cofilin is subject to tight regulation. It is well known that kinase-mediated phosphorylation inactivates ADF/cofilin. Here, however, we found that the activity of Arabidopsis ADF7 is enhanced by CDPK16-mediated phosphorylation. We found that CDPK16 interacts with ADF7 both in vitro and in vivo, and it enhances ADF7-mediated actin depolymerization and severing in vitro in a calcium-dependent manner. Accordingly, the rate of actin turnover is reduced in cdpk16 pollen and the amount of actin filaments increases significantly at the tip of cdpk16 pollen tubes. CDPK16 phosphorylates ADF7 at Serine128 both in vitro and in vivo, and the phospho-mimetic mutant ADF7S128D has enhanced actin-depolymerizing activity compared to ADF7. Strikingly, we found that failure in the phosphorylation of ADF7 at Ser128 impairs its function in promoting actin turnover in vivo, which suggests that this phospho-regulation mechanism is biologically significant. Thus, we reveal that CDPK16-mediated phosphorylation up-regulates ADF7 to promote actin turnover in pollen.
Identifiants
pubmed: 37011088
doi: 10.1371/journal.pbio.3002073
pii: PBIOLOGY-D-21-02463
pmc: PMC10101649
doi:
Substances chimiques
Actin Depolymerizing Factors
0
Actins
0
Arabidopsis Proteins
0
Destrin
0
AT2G17890 protein, Arabidopsis
EC 2.7.11.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e3002073Informations de copyright
Copyright: © 2023 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Annu Rev Plant Physiol Plant Mol Biol. 2000 Jun;51:257-288
pubmed: 15012193
J Cell Biol. 1984 Sep;99(3):769-77
pubmed: 6540783
Proc Natl Acad Sci U S A. 2010 Sep 14;107(37):16390-5
pubmed: 20805480
Plant Cell. 2017 Feb;29(2):395-408
pubmed: 28123105
J Biol Chem. 1971 Aug 10;246(15):4866-71
pubmed: 4254541
J Cell Sci. 1997 Jun;110 ( Pt 11):1269-78
pubmed: 9202387
Cell Motil Cytoskeleton. 1996;35(3):200-9
pubmed: 8913641
Trends Cell Biol. 2010 Apr;20(4):187-95
pubmed: 20133134
J Cell Biol. 1999 Dec 27;147(7):1519-32
pubmed: 10613909
Plant J. 1998 Dec;16(6):735-43
pubmed: 10069079
Mol Biol Cell. 2004 May;15(5):2324-34
pubmed: 15004221
J Mol Biol. 2015 Sep 25;427(19):3137-47
pubmed: 26299936
PLoS Genet. 2018 Nov 12;14(11):e1007789
pubmed: 30418966
Planta. 1998 Mar;204(3):352-9
pubmed: 9530879
J Biol Chem. 1981 Jan 10;256(1):99-105
pubmed: 7005220
Plant Cell. 2003 Jan;15(1):237-49
pubmed: 12509534
Plant Cell Rep. 2013 Nov;32(11):1715-28
pubmed: 23903947
Genome Biol. 2015 Jul 21;16:144
pubmed: 26193878
Plant Cell. 2002 Sep;14(9):2175-90
pubmed: 12215514
Plant Cell. 2010 Aug;22(8):2749-67
pubmed: 20807879
Cell Motil Cytoskeleton. 2000 Dec;47(4):319-36
pubmed: 11093252
Mol Biol Cell. 2001 Aug;12(8):2534-45
pubmed: 11514633
Plant J. 2013 Mar;73(5):747-60
pubmed: 23134061
Mol Plant. 2017 Jul 5;10(7):930-947
pubmed: 28502709
J Biol Chem. 1985 Nov 25;260(27):14410-2
pubmed: 4055781
Trends Cell Biol. 1999 Sep;9(9):364-70
pubmed: 10461190
Chem Biol. 2015 Nov 19;22(11):1461-1469
pubmed: 26526100
J Cell Sci. 2017 Dec 1;130(23):3988-4001
pubmed: 29061882
Nature. 1998 Jun 25;393(6687):805-9
pubmed: 9655397
Plant Cell. 2013 Sep;25(9):3405-23
pubmed: 24058157
Plant J. 2000 Nov;24(4):429-35
pubmed: 11115124
Genome Biol. 2002;3(5):reviews3007
pubmed: 12049672
Plant Physiol. 2008 Feb;146(2):368-76
pubmed: 18065554
Plant Mol Biol. 1998 Jan;36(1):125-36
pubmed: 9484468
Nature. 1998 Jun 25;393(6687):809-12
pubmed: 9655398
Plant J. 1998 Apr;14(2):187-93
pubmed: 9669865
Bio Protoc. 2017 Apr 20;7(8):e2227
pubmed: 34541228
Plant Cell. 2013 May;25(5):1803-17
pubmed: 23715472
FEBS Lett. 2001 Jun 15;499(1-2):97-100
pubmed: 11418120
Nature. 2010 Mar 18;464(7287):418-22
pubmed: 20164835
Science. 1999 Aug 6;285(5429):895-8
pubmed: 10436159
EMBO J. 1999 Dec 1;18(23):6752-61
pubmed: 10581248
Sci China Life Sci. 2020 Feb;63(2):239-250
pubmed: 31240522
Mol Plant. 2015 Dec 7;8(12):1694-709
pubmed: 26433093
J Biol Chem. 1995 Jul 21;270(29):17582-7
pubmed: 7615564
J Cell Biol. 2001 Mar 5;152(5):1019-32
pubmed: 11238457
Biochemistry. 1993 Sep 28;32(38):9985-93
pubmed: 8399167
Plant J. 2008 Jun;54(5):863-75
pubmed: 18298672
J Exp Bot. 2013 Aug;64(11):3327-38
pubmed: 23846874
Science. 2009 Nov 27;326(5957):1208-12
pubmed: 19965462
Plant Cell. 1999 Dec;11(12):2349-63
pubmed: 10590163
Plant Cell. 1994 Dec;6(12):1815-28
pubmed: 7866026
Proc Natl Acad Sci U S A. 2001 Dec 18;98(26):15009-13
pubmed: 11742068
Nat Commun. 2020 Dec 4;11(1):6234
pubmed: 33277490
Mol Cell. 2006 Oct 6;24(1):13-23
pubmed: 17018289
Methods. 2001 Dec;25(4):402-8
pubmed: 11846609
Biochemistry. 1993 Sep 28;32(38):9994-10004
pubmed: 8399168
J Biol Chem. 2003 Nov 7;278(45):44832-42
pubmed: 12947123
Bio Protoc. 2020 Jan 05;10(1):e3509
pubmed: 33654717
Nature. 2017 May 18;545(7654):311-316
pubmed: 28489820
Plant Signal Behav. 2012 Jul;7(7):879-81
pubmed: 22751315
Plant Cell. 2010 Nov;22(11):3745-63
pubmed: 21098731
Plant Cell. 2010 Jun;22(6):1909-35
pubmed: 20543028
Nat Cell Biol. 2001 Mar;3(3):306-10
pubmed: 11231582
Proc Natl Acad Sci U S A. 2019 Jun 11;116(24):12084-12093
pubmed: 31123151
Mol Plant. 2017 Aug 7;10(8):1065-1081
pubmed: 28606871
Planta. 1998 Oct;206(3):435-42
pubmed: 9763711
Plant J. 2000 Dec;24(5):625-36
pubmed: 11123801
Front Cell Dev Biol. 2020 Dec 03;8:614821
pubmed: 33344460
Plant Cell. 2016 Jun;28(6):1422-39
pubmed: 27268429
Plant J. 2015 Aug;83(3):515-27
pubmed: 26096143
PLoS One. 2015 Dec 30;10(12):e0145917
pubmed: 26717562
Plant Cell. 2002 Nov;14(11):2915-27
pubmed: 12417710