OBERON3 and SUPPRESSOR OF MAX2 1-LIKE proteins form a regulatory module driving phloem development.


Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
14 04 2023
Historique:
received: 14 10 2021
accepted: 31 03 2023
medline: 18 4 2023
entrez: 14 4 2023
pubmed: 15 4 2023
Statut: epublish

Résumé

Spatial specificity of cell fate decisions is central for organismal development. The phloem tissue mediates long-distance transport of energy metabolites along plant bodies and is characterized by an exceptional degree of cellular specialization. How a phloem-specific developmental program is implemented is, however, unknown. Here we reveal that the ubiquitously expressed PHD-finger protein OBE3 forms a central module with the phloem-specific SMXL5 protein for establishing the phloem developmental program in Arabidopsis thaliana. By protein interaction studies and phloem-specific ATAC-seq analyses, we show that OBE3 and SMXL5 proteins form a complex in nuclei of phloem stem cells where they promote a phloem-specific chromatin profile. This profile allows expression of OPS, BRX, BAM3, and CVP2 genes acting as mediators of phloem differentiation. Our findings demonstrate that OBE3/SMXL5 protein complexes establish nuclear features essential for determining phloem cell fate and highlight how a combination of ubiquitous and local regulators generate specificity of developmental decisions in plants.

Identifiants

pubmed: 37059727
doi: 10.1038/s41467-023-37790-5
pii: 10.1038/s41467-023-37790-5
pmc: PMC10104830
doi:

Substances chimiques

Arabidopsis Proteins 0
Membrane Proteins 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

2128

Subventions

Organisme : European Research Council
ID : 647148
Pays : International

Informations de copyright

© 2023. The Author(s).

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Auteurs

Eva-Sophie Wallner (ES)

Centre for Organismal Studies (COS), Heidelberg University, 69120, Heidelberg, Germany.
Gilbert Biological Sciences, Stanford University, Stanford, CA, 94305-5020, USA.

Nina Tonn (N)

Centre for Organismal Studies (COS), Heidelberg University, 69120, Heidelberg, Germany.

Dongbo Shi (D)

Centre for Organismal Studies (COS), Heidelberg University, 69120, Heidelberg, Germany.
Japan RIKEN Center for Sustainable Resource Science (CSRS), Yokohama, 230-0045, Japan.
Institute for Biochemistry and Biology (IBB), University of Potsdam, Potsdam, 14476, Germany.
Japan Science and Technology Agency (JST), Saitama, Kawaguchi, Japan.

Laura Luzzietti (L)

Centre for Organismal Studies (COS), Heidelberg University, 69120, Heidelberg, Germany.

Friederike Wanke (F)

Center for Plant Molecular Biology (ZMBP), University of Tübingen, 72076, Tübingen, Germany.

Pascal Hunziker (P)

Centre for Organismal Studies (COS), Heidelberg University, 69120, Heidelberg, Germany.

Yingqiang Xu (Y)

Centre for Organismal Studies (COS), Heidelberg University, 69120, Heidelberg, Germany.

Ilona Jung (I)

Centre for Organismal Studies (COS), Heidelberg University, 69120, Heidelberg, Germany.

Vadir Lopéz-Salmerón (V)

Centre for Organismal Studies (COS), Heidelberg University, 69120, Heidelberg, Germany.
BD Bioscience, 69126, Heidelberg, Germany.

Michael Gebert (M)

Centre for Organismal Studies (COS), Heidelberg University, 69120, Heidelberg, Germany.

Christian Wenzl (C)

Centre for Organismal Studies (COS), Heidelberg University, 69120, Heidelberg, Germany.

Jan U Lohmann (JU)

Centre for Organismal Studies (COS), Heidelberg University, 69120, Heidelberg, Germany.

Klaus Harter (K)

Center for Plant Molecular Biology (ZMBP), University of Tübingen, 72076, Tübingen, Germany.

Thomas Greb (T)

Centre for Organismal Studies (COS), Heidelberg University, 69120, Heidelberg, Germany. thomas.greb@cos.uni-heidelberg.de.

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