Cost of exome analysis in patients with intellectual disability: a micro-costing study in a French setting.

Cost analysis Exome sequencing Intellectual disability Micro-costing

Journal

BMC health services research
ISSN: 1472-6963
Titre abrégé: BMC Health Serv Res
Pays: England
ID NLM: 101088677

Informations de publication

Date de publication:
21 Apr 2023
Historique:
received: 01 07 2022
accepted: 04 04 2023
medline: 25 4 2023
pubmed: 22 4 2023
entrez: 21 04 2023
Statut: epublish

Résumé

With the development of next generation sequencing technologies in France, exome sequencing (ES) has recently emerged as an opportunity to improve the diagnosis rate of patients presenting an intellectual disability (ID). To help French policy makers determine an adequate tariff for ES, we aimed to assess the unit cost per ES diagnostic test for ID from the preparation of the pre-analytical step until the report writing step and to identify its main cost drivers. A micro-costing bottom-up approach was conducted for the year 2018 in a French setting as part of the DISSEQ study, a cost-effectiveness study funded by the Ministry of Health and performed in collaboration with the GAD (Génétique des Anomalies du Développement), a genetic team from the Dijon University Hospital, and a public sequencing platform, the Centre National de Recherche en Génomique Humaine (CNRGH). The analysis was conducted from the point of view of these two ES stakeholders. All of the resources (labor, equipment, disposables and reagents, reusable material) required to analyze blood samples were identified, collected and valued. Several sensitivity analyses were performed. The unit nominal cost per ES diagnostic test for ID was estimated to be €2,019.39. Labor represented 50.7% of the total cost. The analytical step (from the preparation of libraries to the analysis of sequences) represented 88% of the total cost. Sensitivity analyses suggested that a simultaneous price decrease of 20% for the capture kit and 50% for the sequencing support kit led to an estimation of €1,769 per ES diagnostic test for ID. This is the first estimation of ES cost to be done in the French setting of ID diagnosis. The estimation is especially influenced by the price of equipment kits, but more generally by the organization of the centers involved in the different steps of the analysis and the time period in which the study was conducted. This information can now be used to define an adequate tariff and assess the efficiency of ES. ClinicalTrials.gov identifier NCT03287206 on September 19, 2017.

Sections du résumé

BACKGROUND BACKGROUND
With the development of next generation sequencing technologies in France, exome sequencing (ES) has recently emerged as an opportunity to improve the diagnosis rate of patients presenting an intellectual disability (ID). To help French policy makers determine an adequate tariff for ES, we aimed to assess the unit cost per ES diagnostic test for ID from the preparation of the pre-analytical step until the report writing step and to identify its main cost drivers.
METHODS METHODS
A micro-costing bottom-up approach was conducted for the year 2018 in a French setting as part of the DISSEQ study, a cost-effectiveness study funded by the Ministry of Health and performed in collaboration with the GAD (Génétique des Anomalies du Développement), a genetic team from the Dijon University Hospital, and a public sequencing platform, the Centre National de Recherche en Génomique Humaine (CNRGH). The analysis was conducted from the point of view of these two ES stakeholders. All of the resources (labor, equipment, disposables and reagents, reusable material) required to analyze blood samples were identified, collected and valued. Several sensitivity analyses were performed.
RESULTS RESULTS
The unit nominal cost per ES diagnostic test for ID was estimated to be €2,019.39. Labor represented 50.7% of the total cost. The analytical step (from the preparation of libraries to the analysis of sequences) represented 88% of the total cost. Sensitivity analyses suggested that a simultaneous price decrease of 20% for the capture kit and 50% for the sequencing support kit led to an estimation of €1,769 per ES diagnostic test for ID.
CONCLUSION CONCLUSIONS
This is the first estimation of ES cost to be done in the French setting of ID diagnosis. The estimation is especially influenced by the price of equipment kits, but more generally by the organization of the centers involved in the different steps of the analysis and the time period in which the study was conducted. This information can now be used to define an adequate tariff and assess the efficiency of ES.
TRIAL REGISTRATION BACKGROUND
ClinicalTrials.gov identifier NCT03287206 on September 19, 2017.

Identifiants

pubmed: 37085862
doi: 10.1186/s12913-023-09373-z
pii: 10.1186/s12913-023-09373-z
pmc: PMC10120135
doi:

Banques de données

ClinicalTrials.gov
['NCT03287206']

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

386

Subventions

Organisme : French Ministry of Health
ID : PRME-15-0322

Investigateurs

S Arpin (S)
P Blanchet (P)
S Blesson (S)
O Boute-Benejean (O)
T Busa (T)
E Colin (E)
C Coubes (C)
F Devillard (F)
P Edery (P)
S El Chehadeh (S)
M Fradin (M)
A Goldenberg (A)
A-M Guerrot (AM)
Y Herenger (Y)
N Houcinat (N)
N Jean-Marcais (N)
P S Jouk (PS)
L Lambert (L)
A Lavillaureix (A)
M Legendre (M)
B Leheup (B)
S Manouvrier (S)
S Mercier (S)
S Moutton (S)
M Nizon (M)
L Pasquier (L)
F Petit (F)
L Pinson (L)
C Poirsier (C)
L Pons (L)
A Putoux (A)
C Quelin (C)
M Renaud (M)
M Rossi (M)
A Sorlin (A)
M Spodenkiewicz (M)
J Thevenon (J)
A Toutain (A)
J Van-Gils (J)
C Vanlerberghe (C)
A Verloes (A)
M Vincent (M)
C Vincent-Delorme (C)
M Willems (M)
A Ziegler (A)

Informations de copyright

© 2023. The Author(s).

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Auteurs

A L Soilly (AL)

CHU Dijon Bourgogne, Délégation à la Recherche Clinique et à l'Innovation, USMR, F-21000, Dijon, France.
CHU Dijon Bourgogne, Délégation à la Recherche Clinique et à l'Innovation, Unité Innovation, F-21000, Dijon, France.

C Robert-Viard (C)

CHU Dijon Bourgogne, Délégation à la Recherche Clinique et à l'Innovation, Unité Innovation, F-21000, Dijon, France.
CHU Dijon Bourgogne, Inserm, Université de Bourgogne, CIC 1432, Module Épidémiologie Clinique, F21000, Dijon, France.

C Besse (C)

Université Paris-Saclay, CEA, Centre National de Recherche en Génomique Humaine (CNRGH), Evry, France.

A L Bruel (AL)

Inserm, Université Bourgogne-Franche-Comté, UMR1231, équipe GAD, Dijon, France.

B Gerard (B)

Laboratoires de Diagnostic Génétique, Hôpitaux Universitaires de Strasbourg, Institut de Génétique Médicale d'Alsace (IGMA), 67000, Strasbourg, France.

A Boland (A)

Université Paris-Saclay, CEA, Centre National de Recherche en Génomique Humaine (CNRGH), Evry, France.

A Piton (A)

Laboratoires de Diagnostic Génétique, Hôpitaux Universitaires de Strasbourg, Institut de Génétique Médicale d'Alsace (IGMA), 67000, Strasbourg, France.

Y Duffourd (Y)

Inserm, Université Bourgogne-Franche-Comté, UMR1231, équipe GAD, Dijon, France.

J Muller (J)

Laboratoires de Diagnostic Génétique, Hôpitaux Universitaires de Strasbourg, Institut de Génétique Médicale d'Alsace (IGMA), 67000, Strasbourg, France.
Unité Fonctionnelle de Bioinformatique Médicale appliquée au diagnostic (UF7363), Hôpitaux Universitaires de Strasbourg, Strasbourg, France.
Inserm UMRS_1112, Institut de Génétique Médicale d'Alsace, Université de Strasbourg, France et CHRU, Strasbourg, France.

C Poë (C)

Inserm, Université Bourgogne-Franche-Comté, UMR1231, équipe GAD, Dijon, France.

T Jouan (T)

Inserm, Université Bourgogne-Franche-Comté, UMR1231, équipe GAD, Dijon, France.

S El Doueiri (S)

CHU Dijon Bourgogne, Service financier, 21000, Dijon, France.

L Faivre (L)

Inserm, Université Bourgogne-Franche-Comté, UMR1231, équipe GAD, Dijon, France.
CHU Dijon-Bourgogne, Centres de Référence Maladies Rares « Anomalies du Développement et syndromes malformatif de l'Est » et « Déficiences intellectuelles de causes rares », Fédération Hospitalo-Universitaire Médecine Translationnelle et Anomalies du Développement (TRANSLAD), Dijon, France.

D Bacq-Daian (D)

Université Paris-Saclay, CEA, Centre National de Recherche en Génomique Humaine (CNRGH), Evry, France.

B Isidor (B)

Service de Génétique Médicale, CHU de Nantes, Nantes, France.

D Genevieve (D)

Département de Génétique Médicale, Centre de Référence Maladies Rares, Anomalies du Développement et Syndromes Malformatifs Sud-Languedoc Roussillon, Hôpital Arnaud de Villeneuve, Montpellier, France.

S Odent (S)

Service de Génétique Clinique, Centre Hospitalier Universitaire Rennes, F-35203, Rennes, France.
Centre National de la Recherche Scientifique Unité Mixte de Recherche 6290, Institut Génétique et Développement de Rennes, Université de Rennes 1, F-35203, Rennes, France.

N Philip (N)

Département de Génétique Médicale, Hôpital d'Enfants de La Timone, Marseille, France.

M Doco-Fenzy (M)

Service de Génétique, CHU de Reims, EA3801, Reims, France.
CRMR Anddi-Rares constitutif, CLAD-EST, CHU Reims, Reims, France.

D Lacombe (D)

CHU de Bordeaux, Génétique Médicale, INSERM U1211, Laboratoire MRGM, Université de Bordeaux, Bordeaux, France.

M L Asensio (ML)

CHU Dijon Bourgogne, Inserm, Université de Bourgogne, CIC 1432, Module Épidémiologie Clinique, F21000, Dijon, France.

J F Deleuze (JF)

Université Paris-Saclay, CEA, Centre National de Recherche en Génomique Humaine (CNRGH), Evry, France.

C Binquet (C)

CHU Dijon Bourgogne, Inserm, Université de Bourgogne, CIC 1432, Module Épidémiologie Clinique, F21000, Dijon, France.

C Thauvin-Robinet (C)

Inserm, Université Bourgogne-Franche-Comté, UMR1231, équipe GAD, Dijon, France.
CHU Dijon-Bourgogne, Centres de Référence Maladies Rares « Anomalies du Développement et syndromes malformatif de l'Est » et « Déficiences intellectuelles de causes rares », Fédération Hospitalo-Universitaire Médecine Translationnelle et Anomalies du Développement (TRANSLAD), Dijon, France.

C Lejeune (C)

CHU Dijon Bourgogne, Inserm, Université de Bourgogne, CIC 1432, Module Épidémiologie Clinique, F21000, Dijon, France. catherine.lejeune@u-bourgogne.fr.

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