Glycan Stability and Flexibility: Thermodynamic and Kinetic Characterization of Nonconventional Hydrogen Bonding in Lewis Antigens.


Journal

Journal of the American Chemical Society
ISSN: 1520-5126
Titre abrégé: J Am Chem Soc
Pays: United States
ID NLM: 7503056

Informations de publication

Date de publication:
10 05 2023
Historique:
medline: 11 5 2023
pubmed: 26 4 2023
entrez: 26 4 2023
Statut: ppublish

Résumé

We provide evidence for CH-based nonconventional hydrogen bonds (H-bonds) for 10 Lewis antigens and two of their rhamnose analogues. We also characterize the thermodynamics and kinetics of the H-bonds in these molecules and present a plausible explanation for the presence of nonconventional H-bonds in Lewis antigens. Using an alternative method to simultaneously fit a series of temperature-dependent fast exchange nuclear magnetic resonance (NMR) spectra, we determined that the H-bonded conformation is favored by ∼1 kcal/mol over the non-H-bonded conformation. Additionally, a comparison of temperature-dependent

Identifiants

pubmed: 37099481
doi: 10.1021/jacs.2c13104
pmc: PMC10176581
doi:

Substances chimiques

Lewis Blood Group Antigens 0
Rhamnose QN34XC755A
Polysaccharides 0
Hydrogen 7YNJ3PO35Z

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

10022-10034

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Auteurs

Jeahoo Kwon (J)

Division of Bacterial Products and Allergenic Products, Office of Vaccines Research and Review, Center for Biologics Evaluation and Research, Food and Drug Administration, 10903 New Hampshire Avenue, Silver Spring, Maryland 20993, United States.

Alessandro Ruda (A)

Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, S-106 91 Stockholm, Sweden.

Hugo F Azurmendi (HF)

Division of Bacterial Products and Allergenic Products, Office of Vaccines Research and Review, Center for Biologics Evaluation and Research, Food and Drug Administration, 10903 New Hampshire Avenue, Silver Spring, Maryland 20993, United States.

Jasmin Zarb (J)

Division of Bacterial Products and Allergenic Products, Office of Vaccines Research and Review, Center for Biologics Evaluation and Research, Food and Drug Administration, 10903 New Hampshire Avenue, Silver Spring, Maryland 20993, United States.

Marcos D Battistel (MD)

Division of Bacterial Products and Allergenic Products, Office of Vaccines Research and Review, Center for Biologics Evaluation and Research, Food and Drug Administration, 10903 New Hampshire Avenue, Silver Spring, Maryland 20993, United States.

Liang Liao (L)

Department of Chemistry, University of Guelph, Guelph, Ontario N1G 2W1, Canada.

Ari Asnani (A)

Department of Chemistry, University of Guelph, Guelph, Ontario N1G 2W1, Canada.

France-Isabelle Auzanneau (FI)

Department of Chemistry, University of Guelph, Guelph, Ontario N1G 2W1, Canada.

Göran Widmalm (G)

Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, S-106 91 Stockholm, Sweden.

Darón I Freedberg (DI)

Division of Bacterial Products and Allergenic Products, Office of Vaccines Research and Review, Center for Biologics Evaluation and Research, Food and Drug Administration, 10903 New Hampshire Avenue, Silver Spring, Maryland 20993, United States.

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Classifications MeSH