Dynamics of release factor recycling during translation termination in bacteria.
Journal
Nucleic acids research
ISSN: 1362-4962
Titre abrégé: Nucleic Acids Res
Pays: England
ID NLM: 0411011
Informations de publication
Date de publication:
23 06 2023
23 06 2023
Historique:
accepted:
09
04
2023
revised:
05
04
2023
received:
02
07
2022
medline:
26
6
2023
pubmed:
27
4
2023
entrez:
27
4
2023
Statut:
ppublish
Résumé
In bacteria, release of newly synthesized proteins from ribosomes during translation termination is catalyzed by class-I release factors (RFs) RF1 or RF2, reading UAA and UAG or UAA and UGA codons, respectively. Class-I RFs are recycled from the post-termination ribosome by a class-II RF, the GTPase RF3, which accelerates ribosome intersubunit rotation and class-I RF dissociation. How conformational states of the ribosome are coupled to the binding and dissociation of the RFs remains unclear and the importance of ribosome-catalyzed guanine nucleotide exchange on RF3 for RF3 recycling in vivo has been disputed. Here, we profile these molecular events using a single-molecule fluorescence assay to clarify the timings of RF3 binding and ribosome intersubunit rotation that trigger class-I RF dissociation, GTP hydrolysis, and RF3 dissociation. These findings in conjunction with quantitative modeling of intracellular termination flows reveal rapid ribosome-dependent guanine nucleotide exchange to be crucial for RF3 action in vivo.
Identifiants
pubmed: 37102635
pii: 7145697
doi: 10.1093/nar/gkad286
pmc: PMC10287982
doi:
Substances chimiques
Guanosine Triphosphate
86-01-1
Peptide Termination Factors
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
5774-5790Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM113078
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM008294
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM051266
Pays : United States
Informations de copyright
© The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.
Références
Nature. 2019 Sep;573(7775):605-608
pubmed: 31534220
RNA. 2014 May;20(5):609-20
pubmed: 24667215
Nat Chem Biol. 2009 Aug;5(8):593-9
pubmed: 19561621
Mol Cell. 2002 Oct;10(4):789-98
pubmed: 12419223
Nat Commun. 2019 Jun 12;10(1):2579
pubmed: 31189921
Proc Natl Acad Sci U S A. 1994 Jun 21;91(13):5848-52
pubmed: 8016077
RNA. 1999 Aug;5(8):1014-20
pubmed: 10445876
Nature. 2008 Aug 14;454(7206):852-7
pubmed: 18596689
Mol Cell. 1999 May;3(5):601-9
pubmed: 10360176
RNA. 2016 Jan;22(1):10-21
pubmed: 26527791
EMBO J. 1997 Jul 1;16(13):4134-41
pubmed: 9233822
J Mol Biol. 2015 May 8;427(9):1848-60
pubmed: 25619162
Biophys J. 2008 Mar 1;94(5):1826-35
pubmed: 17921203
Biochemistry. 2016 Nov 15;55(45):6344-6354
pubmed: 27779391
Nat Biotechnol. 2016 Jan;34(1):104-10
pubmed: 26641532
Nucleic Acids Res. 2005 Jan 12;33(1):182-9
pubmed: 15647501
J Mol Biol. 1994 May 6;238(3):302-8
pubmed: 8176726
Proc Natl Acad Sci U S A. 2014 Jan 14;111(2):664-9
pubmed: 24379388
Elife. 2013 Jun 04;2:e00411
pubmed: 23755360
Nature. 2011 Jul 31;476(7360):351-4
pubmed: 21804565
EMBO J. 1997 Jul 1;16(13):4126-33
pubmed: 9233821
EMBO J. 1998 Feb 2;17(3):808-16
pubmed: 9451005
Elife. 2019 Sep 12;8:
pubmed: 31513010
PLoS Genet. 2017 Mar 16;13(3):e1006676
pubmed: 28301469
Proc Natl Acad Sci U S A. 2004 Aug 31;101(35):12893-8
pubmed: 15317937
Cell. 2007 Jun 1;129(5):929-41
pubmed: 17540173
Nat Struct Mol Biol. 2017 Sep;24(9):752-757
pubmed: 28741611
Proc Natl Acad Sci U S A. 2008 Dec 16;105(50):19684-9
pubmed: 19064930
Annu Rev Microbiol. 2008;62:353-73
pubmed: 18544041
EMBO J. 2003 Jan 15;22(2):175-82
pubmed: 12514123
Proc Natl Acad Sci U S A. 2008 Oct 7;105(40):15364-9
pubmed: 18824686
Mol Cell. 2006 Jul 21;23(2):183-93
pubmed: 16857585
Proc Natl Acad Sci U S A. 2000 Feb 29;97(5):2046-51
pubmed: 10681447
J Biol Chem. 2021 Jan-Jun;296:100681
pubmed: 33887323
Elife. 2018 Jun 11;7:
pubmed: 29889659
Cell. 2001 Oct 5;107(1):115-24
pubmed: 11595190
Nat Struct Mol Biol. 2004 Oct;11(10):1008-14
pubmed: 15448679
BMC Genomics. 2007 Jan 10;8:15
pubmed: 17214893
Mol Microbiol. 2003 Dec;50(5):1467-76
pubmed: 14651631
Nucleic Acids Res. 2014 Feb;42(3):1812-20
pubmed: 24214994
Mol Genet Genomics. 2015 Aug;290(4):1335-44
pubmed: 25636454
Proc Natl Acad Sci U S A. 1979 Jul;76(7):3174-8
pubmed: 290995
Mol Cell. 2015 Jul 16;59(2):149-61
pubmed: 26186290
Trends Biochem Sci. 2000 Nov;25(11):561-6
pubmed: 11084369
Cell. 2005 Dec 29;123(7):1255-66
pubmed: 16377566
J Mol Biol. 1997 Oct 24;273(2):389-401
pubmed: 9344747
Cell. 2011 Oct 14;147(2):396-408
pubmed: 22000017
EMBO J. 2006 Jun 7;25(11):2539-50
pubmed: 16724118
Mol Biosyst. 2012 Oct 30;8(12):3325-34
pubmed: 23090316
Mol Microbiol. 2003 Jan;47(1):267-75
pubmed: 12492870
Nat Commun. 2018 Aug 3;9(1):3053
pubmed: 30076302
Biochimie. 2008 Mar;90(3):493-9
pubmed: 17999920
Nat Protoc. 2006;1(1):280-5
pubmed: 17406245
Cell Rep. 2017 Jul 5;20(1):161-172
pubmed: 28683310