Structural basis for the ligand promiscuity of the neofunctionalized, carotenoid-binding fasciclin domain protein AstaP.
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
28 04 2023
28 04 2023
Historique:
received:
26
01
2023
accepted:
10
04
2023
medline:
1
5
2023
pubmed:
29
4
2023
entrez:
28
4
2023
Statut:
epublish
Résumé
Fasciclins (FAS1) are ancient adhesion protein domains with no common small ligand binding reported. A unique microalgal FAS1-containing astaxanthin (AXT)-binding protein (AstaP) binds a broad repertoire of carotenoids by a largely unknown mechanism. Here, we explain the ligand promiscuity of AstaP-orange1 (AstaPo1) by determining its NMR structure in complex with AXT and validating this structure by SAXS, calorimetry, optical spectroscopy and mutagenesis. α1-α2 helices of the AstaPo1 FAS1 domain embrace the carotenoid polyene like a jaw, forming a hydrophobic tunnel, too short to cap the AXT β-ionone rings and dictate specificity. AXT-contacting AstaPo1 residues exhibit different conservation in AstaPs with the tentative carotenoid-binding function and in FAS1 proteins generally, which supports the idea of AstaP neofunctionalization within green algae. Intriguingly, a cyanobacterial homolog with a similar domain structure cannot bind carotenoids under identical conditions. These structure-activity relationships provide the first step towards the sequence-based prediction of the carotenoid-binding FAS1 members.
Identifiants
pubmed: 37117801
doi: 10.1038/s42003-023-04832-z
pii: 10.1038/s42003-023-04832-z
pmc: PMC10147662
doi:
Substances chimiques
Carrier Proteins
0
Ligands
0
Cell Adhesion Molecules
0
Carotenoids
36-88-4
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
471Informations de copyright
© 2023. The Author(s).
Références
Biochim Biophys Acta Bioenerg. 2019 May 1;1860(5):414-424
pubmed: 30880081
Biochemistry (Mosc). 2017 Dec;82(13):1592-1614
pubmed: 29523061
Structure. 2003 Feb;11(2):197-203
pubmed: 12575939
J Mol Graph. 1996 Feb;14(1):51-5, 29-32
pubmed: 8744573
Chem Commun (Camb). 2014 Aug 18;50(64):8947-50
pubmed: 24975496
Sci Rep. 2020 Sep 23;10(1):15564
pubmed: 32968135
J Biomol NMR. 2011 Jan;49(1):9-15
pubmed: 21190063
Nucleic Acids Res. 2020 Jul 2;48(W1):W17-W24
pubmed: 32343309
Proc Natl Acad Sci U S A. 2002 Jul 23;99(15):9795-800
pubmed: 12119396
Free Radic Biol Med. 1997;23(7):1086-9
pubmed: 9358253
FEBS Lett. 2009 Apr 17;583(8):1375-80
pubmed: 19328203
Photosynth Res. 2017 Sep;133(1-3):327-341
pubmed: 28213741
Nucleic Acids Res. 2016 Jul 8;44(W1):W344-50
pubmed: 27166375
Structure. 2003 Jan;11(1):55-65
pubmed: 12517340
Mol Plant. 2016 Oct 10;9(10):1379-1394
pubmed: 27392608
Antioxidants (Basel). 2023 Feb 08;12(2):
pubmed: 36829973
J Biomol NMR. 2015 Aug;62(4):453-71
pubmed: 25801209
Proc Natl Acad Sci U S A. 2007 May 22;104(21):8941-6
pubmed: 17496138
J Agric Food Chem. 2000 Apr;48(4):1150-4
pubmed: 10775364
Biochem J. 2002 Feb 15;362(Pt 1):155-64
pubmed: 11829752
Trends Plant Sci. 2020 Jan;25(1):92-104
pubmed: 31679992
Biochim Biophys Acta. 2015 Oct;1847(10):1212-9
pubmed: 25982357
J Appl Crystallogr. 2017 Jun 26;50(Pt 4):1212-1225
pubmed: 28808438
J Biomol NMR. 1993 Jul;3(4):487-93
pubmed: 8400833
Commun Biol. 2018 Aug 27;1:125
pubmed: 30272005
J Cell Sci. 2005 Jul 1;118(Pt 13):2791-801
pubmed: 15976441
J Biomol NMR. 2000 Jan;16(1):23-8
pubmed: 10718609
Nat Commun. 2018 Sep 24;9(1):3869
pubmed: 30250028
J Biol Chem. 2003 Oct 31;278(44):43736-43
pubmed: 12917404
FEBS J. 2022 Feb;289(4):999-1022
pubmed: 34582628
Mol Genet Genomics. 2006 Nov;276(5):478-94
pubmed: 16944204
Commun Biol. 2020 Sep 7;3(1):490
pubmed: 32895456
FEBS Lett. 2018 Jun;592(11):1789-1803
pubmed: 29754429
Arch Biochem Biophys. 2001 Jan 1;385(1):13-9
pubmed: 11361009
Mar Drugs. 2015 May 07;13(5):2857-74
pubmed: 25962124
Cell. 1987 Mar 13;48(5):745-55
pubmed: 3545496
EMBO J. 1994 Sep 15;13(18):4212-22
pubmed: 7925267
Int J Mol Sci. 2018 May 31;19(6):
pubmed: 29857505
Biomol NMR Assign. 2021 Apr;15(1):17-23
pubmed: 32939684
Biol Methods Protoc. 2021 Apr 02;6(1):bpab007
pubmed: 33928191
Biochemistry (Mosc). 2019 Jan;84(Suppl 1):S233-S253
pubmed: 31213205
Plant Cell Physiol. 2013 Jul;54(7):1027-40
pubmed: 23737502
Biochemistry. 1994 May 17;33(19):5984-6003
pubmed: 7514039
Plant Cell. 2001 Apr;13(4):793-806
pubmed: 11283337
FEBS Open Bio. 2013 Jan 11;3:71-7
pubmed: 23772377
FEBS J. 2019 May;286(10):1908-1924
pubmed: 30843329
J Biomol NMR. 2001 Mar;19(3):231-41
pubmed: 11330810
Antioxidants (Basel). 2020 Sep 15;9(9):
pubmed: 32942578
Biotechniques. 1999 Apr;26(4):680-2
pubmed: 10343905
Int J Biol Macromol. 2022 Dec 31;223(Pt A):1381-1393
pubmed: 36395947
J Biomol NMR. 2007 Mar;37(3):205-24
pubmed: 17237975
Phys Chem Chem Phys. 2006 Sep 21;8(35):4053-71
pubmed: 17028694
J Biomol NMR. 1997 Oct;10(3):301-6
pubmed: 20700833
Plant Physiol. 2017 Nov;175(3):1283-1303
pubmed: 28935842
Mar Drugs. 2021 Jun 20;19(6):
pubmed: 34203079
Structure. 2017 Nov 7;25(11):1740-1750.e2
pubmed: 28988748
Photochem Photobiol. 1973 Oct;18(4):263-79
pubmed: 4583619
J Biomol NMR. 2015 Sep;63(1):21-37
pubmed: 26123317
Food Addit Contam. 2006 Nov;23(11):1056-63
pubmed: 17071507
Proc Natl Acad Sci U S A. 2008 Aug 19;105(33):12075-80
pubmed: 18687902
J Magn Reson. 2011 Dec;213(2):423-41
pubmed: 22152361
Protein Expr Purif. 2005 May;41(1):207-34
pubmed: 15915565
Structure. 2022 Dec 1;30(12):1647-1659.e4
pubmed: 36356587