The neighbouring genes AvrLm10A and AvrLm10B are part of a large multigene family of cooperating effector genes conserved in Dothideomycetes and Sordariomycetes.
Brassica napus
Fusarium oxysporum
Leptosphaeria maculans
avirulence
effector family
pathogenic fungi
resistance
Journal
Molecular plant pathology
ISSN: 1364-3703
Titre abrégé: Mol Plant Pathol
Pays: England
ID NLM: 100954969
Informations de publication
Date de publication:
08 2023
08 2023
Historique:
revised:
22
03
2023
received:
27
09
2022
accepted:
26
03
2023
medline:
17
7
2023
pubmed:
2
5
2023
entrez:
2
5
2023
Statut:
ppublish
Résumé
Fungal effectors (small-secreted proteins) have long been considered as species or even subpopulation-specific. The increasing availability of high-quality fungal genomes and annotations has allowed the identification of trans-species or trans-genera families of effectors. Two avirulence effectors, AvrLm10A and AvrLm10B, of Leptosphaeria maculans, the fungus causing stem canker of oilseed rape, are members of such a large family of effectors. AvrLm10A and AvrLm10B are neighbouring genes, organized in divergent transcriptional orientation. Sequence searches within the L. maculans genome showed that AvrLm10A/AvrLm10B belong to a multigene family comprising five pairs of genes with a similar tail-to-tail organization. The two genes, in a pair, always had the same expression pattern and two expression profiles were distinguished, associated with the biotrophic colonization of cotyledons and/or petioles and stems. Of the two protein pairs further investigated, AvrLm10A_like1/AvrLm10B_like1 and AvrLm10A_like2/AvrLm10B_like2, the second one had the ability to physically interact, similarly to what was previously described for the AvrLm10A/AvrLm10B pair, and cross-interactions were also detected for two pairs. AvrLm10A homologues were identified in more than 30 Dothideomycete and Sordariomycete plant-pathogenic fungi. One of them, SIX5, is an effector from Fusarium oxysporum f. sp. lycopersici physically interacting with the avirulence effector Avr2. We found that AvrLm10A/SIX5 homologues were associated with at least eight distinct putative effector families, suggesting that AvrLm10A/SIX5 is able to cooperate with different effectors. These results point to a general role of the AvrLm10A/SIX5 proteins as "cooperating proteins", able to interact with diverse families of effectors whose encoding gene is co-regulated with the neighbouring AvrLm10A homologue.
Identifiants
pubmed: 37128172
doi: 10.1111/mpp.13338
pmc: PMC10346447
doi:
Substances chimiques
Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
914-931Informations de copyright
© 2023 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd.
Références
PLoS Pathog. 2015 Oct 27;11(10):e1005228
pubmed: 26506000
New Phytol. 2013 May;198(3):887-898
pubmed: 23406519
Nat Rev Genet. 2014 Dec;15(12):797-813
pubmed: 25266034
J Biomed Opt. 2003 Jul;8(3):362-7
pubmed: 12880340
Curr Opin Plant Biol. 2021 Aug;62:102054
pubmed: 33992840
Mol Plant Pathol. 2014 Jun;15(5):523-30
pubmed: 24279453
Front Plant Sci. 2015 Nov 17;6:980
pubmed: 26635823
Nucleic Acids Res. 2007 Jul;35(Web Server issue):W645-8
pubmed: 17526519
Mol Biol Evol. 2018 Feb 1;35(2):518-522
pubmed: 29077904
Sci Data. 2018 Nov 06;5:180235
pubmed: 30398473
Annu Rev Plant Biol. 2015;66:513-45
pubmed: 25923844
Nat Methods. 2017 Jun;14(6):587-589
pubmed: 28481363
Mol Plant. 2018 May 7;11(5):691-705
pubmed: 29481865
BMC Biol. 2021 Mar 23;19(1):55
pubmed: 33757516
New Phytol. 2017 Apr;214(2):526-532
pubmed: 28084619
Mol Plant Pathol. 2022 May;23(5):733-748
pubmed: 35239989
Curr Opin Plant Biol. 2020 Aug;56:9-19
pubmed: 32247857
New Phytol. 2017 Nov;216(3):897-914
pubmed: 28857169
Annu Rev Phytopathol. 2018 Aug 25;56:21-40
pubmed: 29768136
New Phytol. 2010 Jan;185(1):285-99
pubmed: 19814776
Mol Plant Microbe Interact. 2006 Dec;19(12):1420-30
pubmed: 17153926
Mol Plant Pathol. 2018 Jul;19(7):1754-1764
pubmed: 29330918
Phytopathology. 2004 Jun;94(6):578-83
pubmed: 18943482
Nat Protoc. 2017 Sep;12(9):1933-1950
pubmed: 28837131
PLoS Pathog. 2022 Jul 6;18(7):e1010664
pubmed: 35793393
PLoS Pathog. 2012;8(11):e1003020
pubmed: 23144620
New Phytol. 2015 Oct;208(2):507-18
pubmed: 25967461
Mol Plant Pathol. 2021 Dec;22(12):1599-1612
pubmed: 34467616
New Phytol. 2019 Jul;223(1):397-411
pubmed: 30802965
New Phytol. 2016 Mar;209(4):1613-24
pubmed: 26592855
Mol Plant Microbe Interact. 2002 Jul;15(7):672-82
pubmed: 12118883
New Phytol. 2021 Aug;231(4):1510-1524
pubmed: 33621369
PLoS Pathog. 2011 Jul;7(7):e1002147
pubmed: 21829350
Plant Cell. 2007 Sep;19(9):2898-912
pubmed: 17873095
Biotechniques. 2002 Jun;32(6):1372-4, 1376, 1378-9
pubmed: 12074169
Mol Plant Microbe Interact. 2018 Jan;31(1):145-162
pubmed: 29144204
Nat Commun. 2011 Feb 15;2:202
pubmed: 21326234
Mol Biol Evol. 2020 May 1;37(5):1530-1534
pubmed: 32011700
Mol Plant Microbe Interact. 2007 Apr;20(4):459-70
pubmed: 17427816
Mol Microbiol. 2009 Feb;71(4):851-63
pubmed: 19170874
Nature. 2006 Nov 16;444(7117):323-9
pubmed: 17108957
Mol Plant Microbe Interact. 2009 Aug;22(8):932-41
pubmed: 19589069
Plant Cell. 2015 Apr;27(4):1332-51
pubmed: 25888589
BMC Genomics. 2014 Oct 12;15:891
pubmed: 25306241
Mol Plant Pathol. 2023 Aug;24(8):914-931
pubmed: 37128172
Science. 2010 Aug 20;329(5994):953-5
pubmed: 20724636
Mol Plant Pathol. 2015 Sep;16(7):699-709
pubmed: 25492575
Mol Microbiol. 2006 Apr;60(1):67-80
pubmed: 16556221
Curr Biol. 2021 Apr 26;31(8):1653-1665.e5
pubmed: 33607033
BMC Genomics. 2012 Dec 11;13:694
pubmed: 23231440
Nature. 2003 Apr 24;422(6934):859-68
pubmed: 12712197
Commun Biol. 2021 Jun 9;4(1):707
pubmed: 34108627