Translation regulation by a guanidine-II riboswitch is highly tunable in sensitivity, dynamic range, and apparent cooperativity.

Riboswitch / genetics Guanidine / pharmacology Ligands Guanidines Aptamers, Nucleotide / chemistry Nucleic Acid Conformation
RelE cooperativity guanidine next-generation sequencing riboswitch translation

Journal

RNA (New York, N.Y.)
ISSN: 1469-9001
Titre abrégé: RNA
Pays: United States
ID NLM: 9509184

Informations de publication

Date de publication:
08 2023
Historique:
received: 18 12 2022
accepted: 05 04 2023
medline: 19 7 2023
pubmed: 3 5 2023
entrez: 2 5 2023
Statut: ppublish

Résumé

Riboswitches function as important translational regulators in bacteria. Comprehensive mutational analysis of transcriptional riboswitches has been used to probe the energetic intricacies of interplay between the aptamer and expression platform, but translational riboswitches have been inaccessible to massively parallel techniques. The guanidine-II (gdm-II) riboswitch is an exclusively translational class. We have integrated RelE cleavage with next-generation sequencing to quantify ligand-dependent changes in translation initiation for all single and double mutations of the

Identifiants

DOI: 10.1261/rna.079560.122 PMID: 37130702 PMC: PMC10351892
pubmed: 37130702
pii: rna.079560.122
doi: 10.1261/rna.079560.122
pmc: PMC10351892
doi:

Substances chimiques

Riboswitch 0
Guanidine JU58VJ6Y3B
Ligands 0
Guanidines 0
Aptamers, Nucleotide 0

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

1126-1139

Subventions

Organisme : NIGMS NIH HHS
ID : R01 GM136969
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM007223
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM067543
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM100884
Pays : United States

Informations de copyright

© 2023 Focht et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society.

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Auteurs

Caroline M Focht (CM)

Institute of Biochemical Design and Discovery, Yale University, West Haven, Connecticut 06516, USA.
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06510, USA.

David A Hiller (DA)

Institute of Biochemical Design and Discovery, Yale University, West Haven, Connecticut 06516, USA.
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06510, USA.

Sabrina G Grunseich (SG)

Institute of Biochemical Design and Discovery, Yale University, West Haven, Connecticut 06516, USA.
Department of Chemistry, Yale University, New Haven, Connecticut 06511, USA.

Scott A Strobel (SA)

Institute of Biochemical Design and Discovery, Yale University, West Haven, Connecticut 06516, USA scott.strobel@yale.edu.
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06510, USA.
Department of Chemistry, Yale University, New Haven, Connecticut 06511, USA.

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Classifications MeSH

questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Séquences d'acides nucléiques régulatrices › Séquences régulatrices de l'acide ribonucléique › Riborégulateur › Translation regulation by a guanidine-II...
questionsmedicales.fr › Composés chimiques organiques › Amidines › Guanidines › Guanidine › Translation regulation by a guanidine-II...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Produits chimiques de laboratoire › Ligands › Translation regulation by a guanidine-II...
questionsmedicales.fr › Composés chimiques organiques › Amidines › Guanidines › Translation regulation by a guanidine-II...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Nucléotides › Polynucléotides › Oligonucléotides › Aptamères nucléotidiques › Translation regulation by a guanidine-II...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Conformation d'acide nucléique › Translation regulation by a guanidine-II...