Leveraging a Structural Blueprint to Rationally Engineer the Rieske Oxygenase TsaM.
Journal
Biochemistry
ISSN: 1520-4995
Titre abrégé: Biochemistry
Pays: United States
ID NLM: 0370623
Informations de publication
Date de publication:
06 06 2023
06 06 2023
Historique:
medline:
7
6
2023
pubmed:
16
5
2023
entrez:
15
5
2023
Statut:
ppublish
Résumé
Rieske nonheme iron oxygenases use two metallocenters, a Rieske-type [2Fe-2S] cluster and a mononuclear iron center, to catalyze oxidation reactions on a broad range of substrates. These enzymes are widely used by microorganisms to degrade environmental pollutants and to build complexity in a myriad of biosynthetic pathways that are industrially interesting. However, despite the value of this chemistry, there is a dearth of understanding regarding the structure-function relationships in this enzyme class, which limits our ability to rationally redesign, optimize, and ultimately exploit the chemistry of these enzymes. Therefore, in this work, by leveraging a combination of available structural information and state-of-the-art protein modeling tools, we show that three "hotspot" regions can be targeted to alter the site selectivity, substrate preference, and substrate scope of the Rieske oxygenase
Identifiants
pubmed: 37188334
doi: 10.1021/acs.biochem.3c00150
pmc: PMC10249351
doi:
Substances chimiques
Oxygenases
EC 1.13.-
Mixed Function Oxygenases
EC 1.-
Dicamba
SJG3M6RY6H
Iron
E1UOL152H7
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1807-1822Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM138271
Pays : United States
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