Identification of Bacteria Associated with Tobacco Mildew and Tobacco-Specific Nitrosamines During Tobacco Fermentation.
Journal
Current microbiology
ISSN: 1432-0991
Titre abrégé: Curr Microbiol
Pays: United States
ID NLM: 7808448
Informations de publication
Date de publication:
19 May 2023
19 May 2023
Historique:
received:
01
08
2022
accepted:
25
04
2023
medline:
22
5
2023
pubmed:
19
5
2023
entrez:
19
5
2023
Statut:
epublish
Résumé
Tobacco mildew and tobacco-specific nitrosamines (TSNAs) affect the quality of tobacco products during fermentation. Microbes are thought to play key roles in the development of specific properties of fermented tobacco; however, little is known about the bacteria involved in the fermentation process. This study aims to identify key microbes related to mildew and TSNA formation. Tobacco was fermented at 25 °C, 35 °C, and 45 °C for 2, 4, and 6 weeks, with unfermented samples used as controls. Our preliminary exploration found that TSNAs content elevated with the increase of temperature and period, and mildew was easy to occur at low temperature with short period. Hence, samples were divided into three groups: the temperature gradient group (25 °C, 35 °C, and 45 °C for 6 weeks); the low-temperature group (control, 25 °C for 2, 4, and 6 weeks); and the high-temperature group (control, 45 °C for 2, 4, and 6 weeks). After collecting fermented tobacco leaves, 16S rRNA gene sequencing was used to explore the structure and dynamic changes of bacterial community during fermentation. Methylobacterium and Deinococcus were shared between the temperature gradient and high-temperature groups and showed a linear downward trend; these might play a role in the production of TSNAs. Massilia, Ruminiclostridium, and Cellulosilyticum species increased with prolonged fermentation time in the low-temperature group; this might be associated with tobacco mildew. In summary, the microbial diversity of fermented tobacco was explored under different conditions. These findings might provide data and material support to improve the quality of fermented tobacco products; however, further omics based studies are warranted to analysis the gene and protein expression patter in the identified bacteria.
Identifiants
pubmed: 37204530
doi: 10.1007/s00284-023-03314-z
pii: 10.1007/s00284-023-03314-z
doi:
Substances chimiques
Nitrosamines
0
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
218Subventions
Organisme : Key Science and Technology Projects of Sichuan Tobacco Company in 2019
ID : SCYC201915
Organisme : Key Science and Technology Projects of Shanghai Tobacco Group in 2019
ID : 2019110004340244
Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
Références
Li Z (2022) Modeling pesticide residues in tobacco leaves for improving life cycle inventory analysis of pesticides in the cigarette industry. Sci Total Environ 845:157267. https://doi.org/10.1016/j.scitotenv.2022.157267
doi: 10.1016/j.scitotenv.2022.157267
pubmed: 35820521
Burki TK (2021) Who releases latest report on the global tobacco epidemic. Lancet Oncol 22(9):1217. https://doi.org/10.1016/S1470-2045(21)00464-2
doi: 10.1016/S1470-2045(21)00464-2
pubmed: 34364410
Yang Y, Peng Q, Ou M, Wu Y, Jing F (2018) Research progress in tobacco fermentation. J Biosci Med 06(6):105–114
Li X, Li M, Wan D, Li L, Chen D, Ding S et al (2019) Role of microorganisms in tobacco fermentation and alcoholization: a review. Microbiol China 46(6):1520–1529
Wang Y (2016) Research progress of microorganism in tobacco fermentation. Sci Res 9:337
Yu J, Li L, Pang T, Ren X, Shao H (2006) Research advancement of tobacco fermentation. J Henan Agric Univ 040(1):108–112
Sun Y (2014) Research progress of microorganism in tobacco fermentation. Mod Agricl Sci Technol 9(12):302–303
Konstantinou E, Fotopoulou F, Drosos A, Dimakopoulou N, Zagoriti Z, Niarchos A et al (2018) Tobacco-specific nitrosamines: a literature review. Food Chem Toxicol 118:198–203
doi: 10.1016/j.fct.2018.05.008
pubmed: 29751076
Sarlak S, Lalou C, Amoedo ND, Rossignol R (2020) Metabolic reprogramming by tobacco-specific nitrosamines (TSNAS) in cancer. Semin Cell Dev Biol 98:154–166
doi: 10.1016/j.semcdb.2019.09.001
pubmed: 31699542
Czoli CD, Hammond D (2018) Trends over time in tobacco-specific nitrosamines (TSNAS) in whole tobacco and smoke emissions from cigarettes sold in Canada. Nicotine Tob Res 20(5):649–653. https://doi.org/10.1093/ntr/ntx103
doi: 10.1093/ntr/ntx103
pubmed: 28595283
Lu J, Zhang H, Su X, Ma D, Du Y (2014) The influence of different storage conditions on the cured tobacco leaves mildew. J Pure Appl Microbiol 8(3):2135–2139
Zhao W (2004) Study on mold and biological control of stored tobacco leaves in Yunnan province. Chinese Academy of Agricultural Sciences, Beijing, China
Zhu G (2005) Identification, biological characteristics and control of moldy organisms in tobacco silos in Guangxi. Guangxi Zhuang Autonomous Region, China
Chen QL, Li Z, Wang HC, Huang Y, Cai LT, Xie HL, Zhou H (2019) Fungal composition and diversity of tobacco phyllosphere from cured tobacco leaves. Acta Microbiol Sin 59(12):2401–2409
Zhang Q, Geng Z, Li D, Ding Z (2020) Characterization and discrimination of microbial community and co-occurrence patterns in fresh and strong flavor style flue-cured tobacco leaves. MicrobiologyOpen 9(2):e965. https://doi.org/10.1002/mbo3.965
doi: 10.1002/mbo3.965
pubmed: 31808296
Li J, Zhao Y, Qin Y, Shi H (2020) Influence of microbiota and metabolites on the quality of tobacco during fermentation. BMC Microbiol 20(1):356. https://doi.org/10.1186/s12866-020-02035-8
doi: 10.1186/s12866-020-02035-8
pubmed: 33213368
pmcid: 7678276
Yang Q, Xiao M, Lin R, Bao X, Pu Y (2014) Research on improving aging quality of tobacco leave by adding enzyme. Southwest China J Agric Sci 27(04):1746–1750
Wen C, Zhang Q, Zhu P, Hu W, Jia Y, Yang S et al (2023) High throughput screening of key functional strains based on improving tobacco quality and mixed fermentation. Front Bioeng Biotechnol 11:1108766. https://doi.org/10.3389/fbioe.2023.1108766
doi: 10.3389/fbioe.2023.1108766
pubmed: 36714011
pmcid: 9880406
Chattopadhyay S, Malayil L, Mongodin EF, Sapkota AR (2021) A roadmap from unknowns to knowns: advancing our understanding of the microbiomes of commercially available tobacco products. Appl Microbiol Biotechnol 105(7):2633–2645. https://doi.org/10.1007/s00253-021-11183-4
doi: 10.1007/s00253-021-11183-4
pubmed: 33704513
pmcid: 7948171
Zhou W, Liu Y, Zeng D, Yang J, Hu X, Song S et al (2016) Research and application on stacking and fermentation technology of cigar tomato core tobacco leaves. Agric Sci 6(3):8
Yang Z, Zhang Z, Song S, Zhang L, Jia L, Chen X et al (2018) Effects of two different fermentation methods on the chemical composition and sensory quality of sun-cured tobacco leaves. J Anhui Agric Sci 46(6):8–11
Yu M, Jia H, Zhou C, Yang Y, Zhao Y, Yang M et al (2017) Variations in gut microbiota and fecal metabolic phenotype associated with depression by 16S rRNA gene sequencing and LC/MS-based metabolomics. J Pharm Biomed Anal 138:231–239
doi: 10.1016/j.jpba.2017.02.008
pubmed: 28219800
Rognes T, Flouri T, Nichols B, Quince C, Mahé F (2016) Vsearch: a versatile open source tool for metagenomics. PeerJ 4:e2584
doi: 10.7717/peerj.2584
pubmed: 27781170
pmcid: 5075697
Leng N, Dawson JA, Thomson JA, Ruotti V, Rissman AI, Smits BM et al (2013) EBSeq: an empirical Bayes hierarchical model for inference in RNA-seq experiments. Bioinformatics (Oxford, England) 29(8):1035–1043
pubmed: 23428641
Schloss PD (2019) Reintroducing mothur: 10 years later. Appl Environ Microbiol 86(2):e02343-19
doi: 10.1128/AEM.02343-19
Aßhauer KP, Bernd W, Rolf D, Peter M (2015) Tax4Fun: predicting functional profiles from metagenomic 16S rRNA data. Bioinformatics (Oxford, England) 31(17):2882–2884
pubmed: 25957349
Wang J, Yang H, Shi H, Zhou J, Bai R, Zhang M et al (2017) Nitrate and nitrite promote formation of tobacco-specific nitrosamines via nitrogen oxides intermediates during postcured storage under warm temperature. J Chem 2017:6135215. https://doi.org/10.1155/2017/6135215
doi: 10.1155/2017/6135215
Li L (2022) Analysis of tobacco leaf modulation and fermentation technology. Agric Henan 598(02):25–26
Liu T, Guo S, Wu C, Zhang R, Zhong Q, Shi H et al (2022) Phyllosphere microbial community of cigar tobacco and its corresponding metabolites. Front Microbiol 13:1025881. https://doi.org/10.3389/fmicb.2022.1025881
doi: 10.3389/fmicb.2022.1025881
pubmed: 36439836
pmcid: 9691965
Zheng T, Zhang Q, Wu Q, Li D, Wu X, Li P et al (2022) Effects of inoculation with acinetobacter on fermentation of cigar tobacco leaves. Front Microbiol 13:911791. https://doi.org/10.3389/fmicb.2022.911791
doi: 10.3389/fmicb.2022.911791
pubmed: 35783443
pmcid: 9248808
Wang R, Shi Z, Zhou J, Bai R, Yang H, Ma Y et al (2014) Changes in TSNAS content during tobacco storage and its response to high temperature treatment. Acta Tabacaria Sinica 20(01):48–53
Sun W, Wang J, Xu D, Zhou J, Bai R, Ma Y et al (2016) Relationship between moisture content of burley tobacco leaves and TSNA formation during high temperature storage. Acta Tabacaria Sinica 22(04):38–43
Zhang Y, Gong W, Liu K, Wang G (2007) Degradation of tobacco-specific nitrosamines by denitrifying bacteria. J Zhongkai Univ Agric Eng 1:5–8
Nestor TB, Gentry JS, Riddick MG, Conner BT, Peele DM, Edwards ME (2003) Role of oxides of nitrogen in tobacco-specific nitrosamine formation in flue-cured tobacco. Beiträge Zur Tabakforschung 20(7):467–475
doi: 10.2478/cttr-2013-0762
Ochsner AM, Sonntag F, Buchhaupt M, Schrader J, Vorholt JA (2015) Methylobacterium extorquens: methylotrophy and biotechnological applications. Appl Microbiol Biotechnol 99(2):517–534
doi: 10.1007/s00253-014-6240-3
pubmed: 25432674
Chistoserdova L (2017) Application of omics approaches to studying Methylotrophs and Methylotroph comunities. Curr Issues Mol Biol 24:119–142
doi: 10.21775/cimb.024.119
pubmed: 28686571
Ardanov P, Sessitsch A, Häggman H, Kozyrovska N, Pirttilä AM (2012) Methylobacterium-induced endophyte community changes correspond with protection of plants against pathogen attack. PLoS ONE 7(10):e46802
doi: 10.1371/journal.pone.0046802
pubmed: 23056459
pmcid: 3463518
Su C, Gu W, Zhe W, Zhang KQ, Duan Y, Yang J (2011) Diversity and phylogeny of bacteria on Zimbabwe tobacco leaves estimated by 16S rRNA sequence analysis. Appl Microbiol Biotechnol 92(5):1033–1044
doi: 10.1007/s00253-011-3367-3
pubmed: 21660545
Chen E, Zhang M, Su J, Sun Z, Zhu K, Zhou Z et al (2019) Prevention and control of mildew of tobacco leaves by regulating air movement in bulk curing barns. J Hunan Univ Arts Sci (Sci Technol) 31(1):56–59+84
Gao Y, Lou X, Chen E, Zhang B, Zhang Z, Shi J et al (2019) Midrib mildew and rot symptoms of tobacco leaves during flue-curing. Tob Sci Technol 52(07):35–40
Yang E, Cui D, Wang W (2019) Research progress on the genus Massilia. Microbiol China 46(6):1537–1548
Lei L, Xia Z, Liu X, Wei HL (2015) Occurrence and variability of tobacco rhizosphere and phyllosphere bacterial communities associated with nicotine biodegradation. Ann Microbiol 65(1):163–173
doi: 10.1007/s13213-014-0847-6
Annan FJ, Al-Sinawi B, Humphreys CM, Norman R, Winzer K, Köpke M et al (2019) Engineering of vitamin prototrophy in Clostridium ljungdahlii and Clostridium autoethanogenum. Appl Microbiol Biotechnol 103(11):4633–4648
doi: 10.1007/s00253-019-09763-6
pubmed: 30972463
pmcid: 6505512
Varel VH (1989) Reisolation and characterization of Clostridium longisporum, a ruminal sporeforming cellulolytic anaerobe. Arch Microbiol 152(3):209–214
doi: 10.1007/BF00409652
pubmed: 2774798
Li S, Shao N, Luo Y, Liu H, Cai S, Dong X (2017) Transcriptome and zymogram analyses reveal a cellobiose-dose related reciprocal regulatory effect on cellulase synthesis in Cellulosilyticum ruminicola H1. Front Microbiol 8:2497
doi: 10.3389/fmicb.2017.02497
pubmed: 29312203
pmcid: 5733062
Ravachol J, Borne R, Meynial-Salles I, Soucaille P, Pagès S, Tardif C et al (2015) Combining free and aggregated cellulolytic systems in the cellulosome-producing bacterium Ruminiclostridium cellulolyticum. Biotechnol Biofuels 8:114
doi: 10.1186/s13068-015-0301-4
pubmed: 26269713
pmcid: 4533799
Guo L, Ma D, Wang J, Ma L (2019) Research progress based on tobacco mildew. Farm Prod Process 101(4):451–461
Bar-Even A, Noor E, Lewis NE, Milo R (2010) Design and analysis of synthetic carbon fixation pathways. Proc Natl Acad Sci USA 107(19):8889–8894
doi: 10.1073/pnas.0907176107
pubmed: 20410460
pmcid: 2889323
Peng Q, Yi T (2007) Analysis of tobacco mildew and its control measures. Chin Agric Sci Bull 023(11):146–150