Knockdown of miR-19a suppresses gastrointestinal dysmotility diarrhea after TBI by regulating VIP expression.

Animals Rats Brain Injuries, Traumatic Diarrhea MicroRNAs / genetics Vasoactive Intestinal Peptide / metabolism
VIP-NO-cGMP-PKG pathway diarrhea miR-19a traumatic brain injury

Journal

Brain and behavior
ISSN: 2162-3279
Titre abrégé: Brain Behav
Pays: United States
ID NLM: 101570837

Informations de publication

Date de publication:
07 2023
Historique:
revised: 27 04 2023
received: 02 12 2022
accepted: 03 05 2023
medline: 14 7 2023
pubmed: 23 5 2023
entrez: 23 5 2023
Statut: ppublish

Résumé

Traumatic brain injury (TBI) is the main cause of death and can lead to a variety of physiological complications, including gastrointestinal dysfunction. The present study aimed to confirm the miR-19a-mediated suppression of diarrhea after TBI through the regulation of VIP expression. A rat model of TBI induced by controlled cortical injury was used to observe gastrointestinal morphology by opening the abdomen after TBI. After 72 h of injury, the fecal water content of the rats was measured. The end ileal segments were removed, and HE staining was used to observe the histopathological changes in the intestine. The levels of serum miR-19a and VIP mRNA were detected by qRT-PCR. ELISA was performed to detect VIP levels in serum. Immunohistochemistry was used to detect the level of VIP in ileal tissues, and immunofluorescence was used to detect c-kit expression in ileal tissue. CCK-8 assay was used to detect the cell viability of interstitial cells of Cajal (ICCs), and TUNEL assay was used to detect apoptosis of ICCs. miR-19a and VIP were highly expressed in the serum of TBI rats, and the knockdown of miR-19a alleviated TBI-induced diarrhea. In addition, the overexpression of miR-19a or VIP inhibited the proliferation of ICCs, promoted apoptosis, and suppressed intracellular Ca Knockdown of miR-19a inhibits activation of the VIP-NO-cGMP-PKG pathway through suppression of VIP expression, which in turn inhibits diarrhea after TBI.

Sections du résumé

BACKGROUND AND AIMS
Traumatic brain injury (TBI) is the main cause of death and can lead to a variety of physiological complications, including gastrointestinal dysfunction. The present study aimed to confirm the miR-19a-mediated suppression of diarrhea after TBI through the regulation of VIP expression.
METHODS
A rat model of TBI induced by controlled cortical injury was used to observe gastrointestinal morphology by opening the abdomen after TBI. After 72 h of injury, the fecal water content of the rats was measured. The end ileal segments were removed, and HE staining was used to observe the histopathological changes in the intestine. The levels of serum miR-19a and VIP mRNA were detected by qRT-PCR. ELISA was performed to detect VIP levels in serum. Immunohistochemistry was used to detect the level of VIP in ileal tissues, and immunofluorescence was used to detect c-kit expression in ileal tissue. CCK-8 assay was used to detect the cell viability of interstitial cells of Cajal (ICCs), and TUNEL assay was used to detect apoptosis of ICCs.
RESULTS
miR-19a and VIP were highly expressed in the serum of TBI rats, and the knockdown of miR-19a alleviated TBI-induced diarrhea. In addition, the overexpression of miR-19a or VIP inhibited the proliferation of ICCs, promoted apoptosis, and suppressed intracellular Ca
CONCLUSION
Knockdown of miR-19a inhibits activation of the VIP-NO-cGMP-PKG pathway through suppression of VIP expression, which in turn inhibits diarrhea after TBI.

Identifiants

DOI: 10.1002/brb3.3071 PMID: 37218372 PMC: PMC10338784
pubmed: 37218372
doi: 10.1002/brb3.3071
pmc: PMC10338784
doi:

Substances chimiques

MicroRNAs 0
Vasoactive Intestinal Peptide 37221-79-7

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e3071

Informations de copyright

© 2023 The Authors. Brain and Behavior published by Wiley Periodicals LLC.

Références

Am J Physiol Gastrointest Liver Physiol. 2018 May 1;314(5):G610-G622
pubmed: 29420068
Probiotics Antimicrob Proteins. 2017 Jun;9(2):172-181
pubmed: 28303478
Int J Mol Sci. 2018 Mar 25;19(4):
pubmed: 29587404
Neurogastroenterol Motil. 2013 Jul;25(7):593-e463
pubmed: 23551971
Gut. 2019 Dec;68(12):2238-2250
pubmed: 31563877
Regul Pept. 2008 Nov 29;151(1-3):75-9
pubmed: 18926858
Biophys J. 2019 Aug 20;117(4):767-779
pubmed: 31400920
Evid Based Complement Alternat Med. 2021 Jul 27;2021:5596531
pubmed: 34354758
Cell Physiol Biochem. 2018;46(5):2127-2137
pubmed: 29723849
Aging (Albany NY). 2020 Nov 4;12(21):21687-21705
pubmed: 33147167
Indian J Gastroenterol. 2021 Apr;40(2):198-208
pubmed: 33792838
Drug Des Devel Ther. 2018 Aug 01;12:2403-2411
pubmed: 30122898
World J Gastroenterol. 2017 Aug 7;23(29):5313-5323
pubmed: 28839431
Braz J Med Biol Res. 2018 Oct 04;51(11):e7541
pubmed: 30304131
Am J Physiol Gastrointest Liver Physiol. 2001 May;280(5):G787-94
pubmed: 11292585
Dig Dis Sci. 2018 Mar;63(3):619-627
pubmed: 29372479
Int J Mol Med. 2018 Oct;42(4):2120-2128
pubmed: 30015849
Am J Physiol Gastrointest Liver Physiol. 2017 Nov 1;313(5):G361-G372
pubmed: 28774868
Hepatogastroenterology. 2009 Sep-Oct;56(94-95):1403-6
pubmed: 19950799
Brain Behav. 2023 Jul;13(7):e3071
pubmed: 37218372
Small. 2022 May;18(18):e2200302
pubmed: 35384294
Sex Med. 2021 Aug;9(4):100390
pubmed: 34246178
J Neurotrauma. 2001 Dec;18(12):1373-81
pubmed: 11780867
Sci Rep. 2016 Jun 24;6:28148
pubmed: 27338832
J Neurogastroenterol Motil. 2018 Jan 30;24(1):128-137
pubmed: 28774158
Metab Brain Dis. 2018 Oct;33(5):1755-1760
pubmed: 30014176
Ann Surg. 2017 Aug;266(2):346-352
pubmed: 27501174
Nat Commun. 2020 Sep 22;11(1):4791
pubmed: 32963229
Evid Based Complement Alternat Med. 2017;2017:4249016
pubmed: 29403536
J Neuroinflammation. 2022 Mar 28;19(1):71
pubmed: 35346242
Results Probl Cell Differ. 2010;50:221-34
pubmed: 19859678
Orphanet J Rare Dis. 2019 Feb 8;14(1):33
pubmed: 30736844
Nutr Neurosci. 2022 Mar;25(3):530-536
pubmed: 32431234
BMJ Case Rep. 2017 Jul 27;2017:
pubmed: 28751427
Int J Mol Sci. 2020 Jun 25;21(12):
pubmed: 32630607
Neurogastroenterol Motil. 2004 Apr;16 Suppl 1:118-22
pubmed: 15066016
Biosci Rep. 2018 Oct 17;38(5):
pubmed: 30232232
Cureus. 2022 Mar 3;14(3):e22819
pubmed: 35273891
PLoS One. 2015 Jun 01;10(6):e0128214
pubmed: 26030918
J Cell Mol Med. 2021 Apr;25(8):3754-3764
pubmed: 33629528
Evid Based Complement Alternat Med. 2020 Mar 31;2020:3575231
pubmed: 32328126
Chin J Integr Med. 2020 Oct;26(10):762-768
pubmed: 31446576
ASN Neuro. 2021 Jan-Dec;13:1759091420984920
pubmed: 33430619
Exp Ther Med. 2020 Dec;20(6):155
pubmed: 33093893
World J Gastroenterol. 2004 Mar 15;10(6):875-80
pubmed: 15040036
Gastroenterology. 2021 Apr;160(5):1662-1678.e18
pubmed: 33421511
Mol Cells. 2006 Jun 30;21(3):337-42
pubmed: 16819295
Exp Ther Med. 2018 Jun;15(6):4875-4879
pubmed: 29805508

Auteurs

Ying An (Y)

Department of Gastroenterology, The First People's Hospital of Yunnan Province/The Affiliated Hospital of Kunming University of Science and Technology, Kunming, Yunnan, China.

Sheng Hu (S)

Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Kunming Medical University, Kunming, China.

Yu Zhang (Y)

Department of Gastroenterology, The First People's Hospital of Yunnan Province/The Affiliated Hospital of Kunming University of Science and Technology, Kunming, Yunnan, China.

Zhengji Song (Z)

Department of Gastroenterology, The First People's Hospital of Yunnan Province/The Affiliated Hospital of Kunming University of Science and Technology, Kunming, Yunnan, China.

Ruochang Li (R)

Department of Gastroenterology, The First People's Hospital of Yunnan Province/The Affiliated Hospital of Kunming University of Science and Technology, Kunming, Yunnan, China.

Yan Li (Y)

Department of Gastroenterology, The First People's Hospital of Yunnan Province/The Affiliated Hospital of Kunming University of Science and Technology, Kunming, Yunnan, China.

Yongli Li (Y)

Department of Gastroenterology, The First People's Hospital of Yunnan Province/The Affiliated Hospital of Kunming University of Science and Technology, Kunming, Yunnan, China.

Wenjun Ren (W)

Department of Thoracic Surgery, The First People's Hospital of Yunnan Province/The Affiliated Hospital of Kunming University of Science and Technology, Kunming, Yunnan, China.

Ping Wan (P)

Department of Gastroenterology, The First People's Hospital of Yunnan Province/The Affiliated Hospital of Kunming University of Science and Technology, Kunming, Yunnan, China.
ORCID: 0000-0003-3078-7580

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questionsmedicales.fr › Eucaryotes › Animaux › Knockdown of miR-19a suppresses...
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questionsmedicales.fr › Plaies et blessures › Traumatismes du système nerveux › Traumatismes cranioencéphaliques › Lésions encéphaliques › Lésions traumatiques de l'encéphale › Knockdown of miR-19a suppresses...
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questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines de tissu nerveux › Neuropeptides › Peptide vasoactif intestinal › Knockdown of miR-19a suppresses...