Crystal structure of thermostable acetaldehyde dehydrogenase from the hyperthermophilic archaeon Sulfolobus tokodaii.
Sulfolobus tokodaii
acetaldehyde dehydrogenase
aldehydes
archaea
thermostability
Journal
Acta crystallographica. Section F, Structural biology communications
ISSN: 2053-230X
Titre abrégé: Acta Crystallogr F Struct Biol Commun
Pays: United States
ID NLM: 101620319
Informations de publication
Date de publication:
01 Jun 2023
01 Jun 2023
Historique:
received:
15
02
2023
accepted:
22
05
2023
pmc-release:
01
06
2024
medline:
2
6
2023
pubmed:
25
5
2023
entrez:
25
5
2023
Statut:
ppublish
Résumé
Aldehyde dehydrogenase (ALDH) is widely distributed in nature and its characteristics have been examined. ALDH plays an important role in aldehyde detoxification. Sources of aldehydes include incomplete combustion and emissions from paints, linoleum and varnishes in the living environment. Acetaldehyde is also considered to be carcinogenic and toxic. Thermostable ALDH from the hyperthermophilic archaeon Sulfolobus tokodaii exhibits high activity towards acetaldehyde and has potential applications as a biosensor for acetaldehyde. Thermostable ALDH displays a unique and wide adaptability. Therefore, its crystal structure can provide new insights into the catalytic mechanism and potential applications of ALDHs. However, a crystal structure of a thermostable ALDH exhibiting high activity towards acetaldehyde has not been reported to date. In this study, crystals of recombinant thermostable ALDH from S. tokodaii were prepared and the crystal structure of its holo form was determined. A crystal of the enzyme was prepared and its structure in complex with NADP was determined at a resolution of 2.2 Å. This structural analysis may facilitate further studies on catalytic mechanisms and applications.
Identifiants
pubmed: 37227376
pii: S2053230X23004430
doi: 10.1107/S2053230X23004430
pmc: PMC10231261
doi:
Substances chimiques
aldehyde dehydrogenase (NAD(P)+)
EC 1.2.1.5
Acetaldehyde
GO1N1ZPR3B
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
159-165Références
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