CRISPR-Based Genome Editing Tools: An Accelerator in Crop Breeding for a Changing Future.


Journal

International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791

Informations de publication

Date de publication:
11 May 2023
Historique:
received: 03 02 2023
revised: 03 05 2023
accepted: 04 05 2023
medline: 29 5 2023
pubmed: 27 5 2023
entrez: 27 5 2023
Statut: epublish

Résumé

Genome editing is an important strategy to maintain global food security and achieve sustainable agricultural development. Among all genome editing tools, CRISPR-Cas is currently the most prevalent and offers the most promise. In this review, we summarize the development of CRISPR-Cas systems, outline their classification and distinctive features, delineate their natural mechanisms in plant genome editing and exemplify the applications in plant research. Both classical and recently discovered CRISPR-Cas systems are included, detailing the class, type, structures and functions of each. We conclude by highlighting the challenges that come with CRISPR-Cas and offer suggestions on how to tackle them. We believe the gene editing toolbox will be greatly enriched, providing new avenues for a more efficient and precise breeding of climate-resilient crops.

Identifiants

pubmed: 37239967
pii: ijms24108623
doi: 10.3390/ijms24108623
pmc: PMC10218198
pii:
doi:

Types de publication

Journal Article Review

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : Australian Research Council
ID : DP210100296
Organisme : Australian Research Council
ID : DP200100762

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Auteurs

Fangning Zhang (F)

College of Life Sciences, Shandong Normal University, Jinan 250014, China.

Ting Xiang Neik (TX)

School of Biosciences, University of Nottingham Malaysia, Semenyih 43500, Malaysia.

William J W Thomas (WJW)

School of Biological Sciences, University of Western Australia, Perth, WA 6009, Australia.

Jacqueline Batley (J)

School of Biological Sciences, Institute of Agriculture, University of Western Australia, Perth, WA 6009, Australia.

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Classifications MeSH